Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25990 | 5' | -59.1 | NC_005340.1 | + | 20993 | 0.65 | 0.405739 |
Target: 5'- aCCGUCGUCagcgccaGCAcguUAUCGGCCucaCCGGCc -3' miRNA: 3'- -GGCGGCAG-------CGUu--AUGGCUGG---GGCCGu -5' |
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25990 | 5' | -59.1 | NC_005340.1 | + | 16232 | 0.66 | 0.387414 |
Target: 5'- aCCGCCGcCGCGAUAguuuUCGAgCUugcgggucaguuuCGGCAg -3' miRNA: 3'- -GGCGGCaGCGUUAU----GGCUgGG-------------GCCGU- -5' |
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25990 | 5' | -59.1 | NC_005340.1 | + | 5331 | 0.66 | 0.378458 |
Target: 5'- -gGCUGgcuGCAaaaGUACCGcaaugaaGCCCCGGCGc -3' miRNA: 3'- ggCGGCag-CGU---UAUGGC-------UGGGGCCGU- -5' |
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25990 | 5' | -59.1 | NC_005340.1 | + | 12258 | 0.67 | 0.312722 |
Target: 5'- aCCGCCcggcauggugGcCGCAGcaagcuUAgCGGCUCCGGCAg -3' miRNA: 3'- -GGCGG----------CaGCGUU------AUgGCUGGGGCCGU- -5' |
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25990 | 5' | -59.1 | NC_005340.1 | + | 20533 | 0.67 | 0.311948 |
Target: 5'- aCCGCauaucgaccaGUCGCAGgaauCCGACgcgguauuucuuaCCCGGCu -3' miRNA: 3'- -GGCGg---------CAGCGUUau--GGCUG-------------GGGCCGu -5' |
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25990 | 5' | -59.1 | NC_005340.1 | + | 20153 | 0.68 | 0.297515 |
Target: 5'- aUGCCGgUGCAAgACUGGCaCCGGCGu -3' miRNA: 3'- gGCGGCaGCGUUaUGGCUGgGGCCGU- -5' |
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25990 | 5' | -59.1 | NC_005340.1 | + | 7098 | 0.69 | 0.242431 |
Target: 5'- gCGCUaUCGCGGUGuuGaaGCCaCCGGCAa -3' miRNA: 3'- gGCGGcAGCGUUAUggC--UGG-GGCCGU- -5' |
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25990 | 5' | -59.1 | NC_005340.1 | + | 28317 | 0.7 | 0.21822 |
Target: 5'- gCCGCCGUC-CAugAUGCCGcugACgCCGGUg -3' miRNA: 3'- -GGCGGCAGcGU--UAUGGC---UGgGGCCGu -5' |
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25990 | 5' | -59.1 | NC_005340.1 | + | 63 | 0.7 | 0.206908 |
Target: 5'- aCCGCUGgcgCGCAAUGCUuuCCCCGcCAc -3' miRNA: 3'- -GGCGGCa--GCGUUAUGGcuGGGGCcGU- -5' |
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25990 | 5' | -59.1 | NC_005340.1 | + | 8198 | 0.71 | 0.175975 |
Target: 5'- gCGCUGgcCGUA--ACCGGCgCCCGGCAa -3' miRNA: 3'- gGCGGCa-GCGUuaUGGCUG-GGGCCGU- -5' |
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25990 | 5' | -59.1 | NC_005340.1 | + | 5643 | 0.72 | 0.149226 |
Target: 5'- cCUGCUGUgCGC-GUGCCGuacuUCCCGGCGa -3' miRNA: 3'- -GGCGGCA-GCGuUAUGGCu---GGGGCCGU- -5' |
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25990 | 5' | -59.1 | NC_005340.1 | + | 16853 | 0.73 | 0.137285 |
Target: 5'- aCGCCG-CGCAggGUACCGGCgUUCGGCu -3' miRNA: 3'- gGCGGCaGCGU--UAUGGCUG-GGGCCGu -5' |
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25990 | 5' | -59.1 | NC_005340.1 | + | 17767 | 1.1 | 0.000192 |
Target: 5'- cCCGCCGUCGCAAUACCGACCCCGGCAg -3' miRNA: 3'- -GGCGGCAGCGUUAUGGCUGGGGCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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