Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25991 | 3' | -46.8 | NC_005340.1 | + | 12631 | 0.67 | 0.90262 |
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Target: 5'- gCGUCGCCAUCACCaucgUUGaguCAAUGGa- -3' miRNA: 3'- -GUAGUGGUGGUGGaa--AAC---GUUACUau -5' |
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| 25991 | 3' | -46.8 | NC_005340.1 | + | 13403 | 0.67 | 0.90262 |
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Target: 5'- aAUU-CCACCACCUgaugUUGUggUGGg- -3' miRNA: 3'- gUAGuGGUGGUGGAa---AACGuuACUau -5' |
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| 25991 | 3' | -46.8 | NC_005340.1 | + | 5727 | 0.67 | 0.90262 |
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Target: 5'- ---aGCCACCGCCgaa-GCAuUGAUGa -3' miRNA: 3'- guagUGGUGGUGGaaaaCGUuACUAU- -5' |
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| 25991 | 3' | -46.8 | NC_005340.1 | + | 20318 | 0.68 | 0.869218 |
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Target: 5'- gCGUCAgCACCGCCccgcGCAggGGUAa -3' miRNA: 3'- -GUAGUgGUGGUGGaaaaCGUuaCUAU- -5' |
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| 25991 | 3' | -46.8 | NC_005340.1 | + | 10588 | 0.68 | 0.869218 |
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Target: 5'- --aCGCCACCAUgaUUUGCAGccUGAg- -3' miRNA: 3'- guaGUGGUGGUGgaAAACGUU--ACUau -5' |
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| 25991 | 3' | -46.8 | NC_005340.1 | + | 4758 | 0.69 | 0.837853 |
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Target: 5'- uCAUCGCCACCACCaccgcuggcuuucgUGCGucGUGGc- -3' miRNA: 3'- -GUAGUGGUGGUGGaaa-----------ACGU--UACUau -5' |
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| 25991 | 3' | -46.8 | NC_005340.1 | + | 3489 | 0.7 | 0.776883 |
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Target: 5'- aGUCGCCGCCugACUUUUgcggcuugGCGAUGAg- -3' miRNA: 3'- gUAGUGGUGG--UGGAAAa-------CGUUACUau -5' |
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| 25991 | 3' | -46.8 | NC_005340.1 | + | 23683 | 0.7 | 0.776883 |
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Target: 5'- aAUCACCAagcuCCUUUUGCA-UGGUAa -3' miRNA: 3'- gUAGUGGUggu-GGAAAACGUuACUAU- -5' |
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| 25991 | 3' | -46.8 | NC_005340.1 | + | 10122 | 0.79 | 0.303745 |
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Target: 5'- cCAUUACCGCCACCg--UGcCGGUGGUAc -3' miRNA: 3'- -GUAGUGGUGGUGGaaaAC-GUUACUAU- -5' |
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| 25991 | 3' | -46.8 | NC_005340.1 | + | 17974 | 1.08 | 0.003622 |
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Target: 5'- gCAUCACCACCACCUUUUGCAAUGAUAa -3' miRNA: 3'- -GUAGUGGUGGUGGAAAACGUUACUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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