Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25992 | 3' | -55 | NC_005340.1 | + | 7493 | 0.65 | 0.566247 |
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Target: 5'- --gGCACUGAuUGUGGUCGgAAaagugcUGGCa -3' miRNA: 3'- uagCGUGGCUcACGCCAGUgUU------ACCG- -5' |
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| 25992 | 3' | -55 | NC_005340.1 | + | 18974 | 0.66 | 0.52118 |
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Target: 5'- -cCGCACCGA---CGGcCACAAUcGGCc -3' miRNA: 3'- uaGCGUGGCUcacGCCaGUGUUA-CCG- -5' |
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| 25992 | 3' | -55 | NC_005340.1 | + | 14647 | 0.66 | 0.510124 |
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Target: 5'- -cCGCGCCa---GCGGU-GCAAUGGCu -3' miRNA: 3'- uaGCGUGGcucaCGCCAgUGUUACCG- -5' |
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| 25992 | 3' | -55 | NC_005340.1 | + | 15746 | 0.67 | 0.488316 |
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Target: 5'- cUCGCACCGc--GCaGGUacuggccgaCACGAUGGCc -3' miRNA: 3'- uAGCGUGGCucaCG-CCA---------GUGUUACCG- -5' |
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| 25992 | 3' | -55 | NC_005340.1 | + | 20787 | 0.67 | 0.477577 |
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Target: 5'- uGUUaCACCG-GUauaagcuccGCGGUCGgCAAUGGCa -3' miRNA: 3'- -UAGcGUGGCuCA---------CGCCAGU-GUUACCG- -5' |
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| 25992 | 3' | -55 | NC_005340.1 | + | 98 | 0.68 | 0.405978 |
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Target: 5'- uUCGUugaugAUCGGGUGUGGUUguGCGcguGUGGCg -3' miRNA: 3'- uAGCG-----UGGCUCACGCCAG--UGU---UACCG- -5' |
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| 25992 | 3' | -55 | NC_005340.1 | + | 2476 | 0.68 | 0.396309 |
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Target: 5'- cUCGCGCCGccgGUGGUUucgGGUGGCa -3' miRNA: 3'- uAGCGUGGCucaCGCCAGug-UUACCG- -5' |
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| 25992 | 3' | -55 | NC_005340.1 | + | 12859 | 0.73 | 0.21104 |
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Target: 5'- -gCGaCACCGAaaGCGGUCAaGGUGGCa -3' miRNA: 3'- uaGC-GUGGCUcaCGCCAGUgUUACCG- -5' |
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| 25992 | 3' | -55 | NC_005340.1 | + | 15549 | 0.77 | 0.108537 |
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Target: 5'- -aCGCagGCCGAGUGCacgCGCAGUGGCg -3' miRNA: 3'- uaGCG--UGGCUCACGccaGUGUUACCG- -5' |
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| 25992 | 3' | -55 | NC_005340.1 | + | 18946 | 1.1 | 0.00035 |
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Target: 5'- aAUCGCACCGAGUGCGGUCACAAUGGCa -3' miRNA: 3'- -UAGCGUGGCUCACGCCAGUGUUACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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