miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
25992 5' -47.4 NC_005340.1 + 22440 0.66 0.938684
Target:  5'- aCAGCAGUAuuuaGAGGCCGUuuUCAAUgagcuaaaGCGg -3'
miRNA:   3'- -GUUGUCAU----UUCUGGCAugGGUUG--------UGC- -5'
25992 5' -47.4 NC_005340.1 + 28293 0.67 0.926752
Target:  5'- -cACGGU--AGACCGU-CaCCGGCGCa -3'
miRNA:   3'- guUGUCAuuUCUGGCAuG-GGUUGUGc -5'
25992 5' -47.4 NC_005340.1 + 9378 0.67 0.920285
Target:  5'- cCAGuCGGUGcgaaguaagaAAGGCCGUAUCaGGCGCGa -3'
miRNA:   3'- -GUU-GUCAU----------UUCUGGCAUGGgUUGUGC- -5'
25992 5' -47.4 NC_005340.1 + 8229 0.7 0.807756
Target:  5'- aGACAGUGaccagcuuuucGAGACUGUGCUC-GCGCu -3'
miRNA:   3'- gUUGUCAU-----------UUCUGGCAUGGGuUGUGc -5'
25992 5' -47.4 NC_005340.1 + 6267 0.7 0.7644
Target:  5'- --uCAGUGguGAGGCCGUAUUgCAGCGCGu -3'
miRNA:   3'- guuGUCAU--UUCUGGCAUGG-GUUGUGC- -5'
25992 5' -47.4 NC_005340.1 + 16650 0.72 0.687582
Target:  5'- cCAGCAGUGcuGACCGgucagggaauuacaGCCCGauuGCGCGg -3'
miRNA:   3'- -GUUGUCAUuuCUGGCa-------------UGGGU---UGUGC- -5'
25992 5' -47.4 NC_005340.1 + 18912 1.09 0.0035
Target:  5'- gCAACAGUAAAGACCGUACCCAACACGc -3'
miRNA:   3'- -GUUGUCAUUUCUGGCAUGGGUUGUGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.