Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25995 | 3' | -51.9 | NC_005340.1 | + | 6830 | 0.66 | 0.739458 |
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Target: 5'- aCGGGAAucaCCACGCCCGGuacGUCcuGCGGu -3' miRNA: 3'- cGUUUUUc--GGUGCGGGCCu--UAG--CGUC- -5' |
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| 25995 | 3' | -51.9 | NC_005340.1 | + | 21140 | 0.66 | 0.739458 |
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Target: 5'- cGCuuAAAGCCugacacGCGCaCCGGuGUCuCAGg -3' miRNA: 3'- -CGuuUUUCGG------UGCG-GGCCuUAGcGUC- -5' |
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| 25995 | 3' | -51.9 | NC_005340.1 | + | 16373 | 0.67 | 0.68206 |
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Target: 5'- aGCAAAAcgcagcggcacAGCaucaGCGCCCGGGGcggCGUAc -3' miRNA: 3'- -CGUUUU-----------UCGg---UGCGGGCCUUa--GCGUc -5' |
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| 25995 | 3' | -51.9 | NC_005340.1 | + | 13160 | 0.67 | 0.670362 |
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Target: 5'- gGCGGAGuuuaucGCCugGCUgGaGAGUUGCGGc -3' miRNA: 3'- -CGUUUUu-----CGGugCGGgC-CUUAGCGUC- -5' |
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| 25995 | 3' | -51.9 | NC_005340.1 | + | 3666 | 0.7 | 0.474647 |
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Target: 5'- aGUAAAGcAGCgAUGCCUGucGUCGCGGg -3' miRNA: 3'- -CGUUUU-UCGgUGCGGGCcuUAGCGUC- -5' |
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| 25995 | 3' | -51.9 | NC_005340.1 | + | 2898 | 0.71 | 0.442723 |
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Target: 5'- cGCAGAugcuuuugcgAGGCCAUGCCCGaGGcgacuuugCGCAGc -3' miRNA: 3'- -CGUUU----------UUCGGUGCGGGC-CUua------GCGUC- -5' |
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| 25995 | 3' | -51.9 | NC_005340.1 | + | 9749 | 0.72 | 0.392341 |
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Target: 5'- uGCAAGGAuGCcuCugGUCUGGAGUCGCGc -3' miRNA: 3'- -CGUUUUU-CG--GugCGGGCCUUAGCGUc -5' |
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| 25995 | 3' | -51.9 | NC_005340.1 | + | 25151 | 0.72 | 0.38272 |
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Target: 5'- uGCAuuacGGCCACGgCUGGA-UUGCAGg -3' miRNA: 3'- -CGUuuu-UCGGUGCgGGCCUuAGCGUC- -5' |
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| 25995 | 3' | -51.9 | NC_005340.1 | + | 10829 | 0.88 | 0.032173 |
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Target: 5'- aGCAGGAAGCCAuCGCCCGGAcgcUCGCAc -3' miRNA: 3'- -CGUUUUUCGGU-GCGGGCCUu--AGCGUc -5' |
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| 25995 | 3' | -51.9 | NC_005340.1 | + | 26101 | 1.11 | 0.000745 |
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Target: 5'- cGCAAAAAGCCACGCCCGGAAUCGCAGc -3' miRNA: 3'- -CGUUUUUCGGUGCGGGCCUUAGCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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