Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25996 | 3' | -59.4 | NC_005340.1 | + | 25248 | 0.66 | 0.346437 |
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Target: 5'- aGCCuGUGaGCGGCUagGGUGcCAGCGc -3' miRNA: 3'- -UGG-CACgCGCCGGagCCAUaGUCGCu -5' |
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| 25996 | 3' | -59.4 | NC_005340.1 | + | 16810 | 0.67 | 0.283631 |
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Target: 5'- uACCcUGCGCGGCgUCaGUcuggCGGCGGu -3' miRNA: 3'- -UGGcACGCGCCGgAGcCAua--GUCGCU- -5' |
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| 25996 | 3' | -59.4 | NC_005340.1 | + | 2498 | 0.69 | 0.23018 |
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Target: 5'- gACCGgGCGUGaGCCgaaUGGaugGUCGGCGAa -3' miRNA: 3'- -UGGCaCGCGC-CGGa--GCCa--UAGUCGCU- -5' |
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| 25996 | 3' | -59.4 | NC_005340.1 | + | 7634 | 0.71 | 0.157161 |
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Target: 5'- cGCCGUGUGCGGUauuggauccaugCUCGGUAUUGcCGGu -3' miRNA: 3'- -UGGCACGCGCCG------------GAGCCAUAGUcGCU- -5' |
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| 25996 | 3' | -59.4 | NC_005340.1 | + | 18922 | 0.71 | 0.152838 |
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Target: 5'- gGCCGauUGUGGCCgUCGGUG-CGGCGAu -3' miRNA: 3'- -UGGCacGCGCCGG-AGCCAUaGUCGCU- -5' |
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| 25996 | 3' | -59.4 | NC_005340.1 | + | 28332 | 1.08 | 0.000201 |
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Target: 5'- gACCGUGCGCGGCCUCGGUAUCAGCGAg -3' miRNA: 3'- -UGGCACGCGCCGGAGCCAUAGUCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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