Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25996 | 5' | -56.6 | NC_005340.1 | + | 20332 | 0.66 | 0.479715 |
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Target: 5'- uUUUACGGUcgaugAGAUUGagCACCGGgGCGc -3' miRNA: 3'- uAAGUGCCA-----UCUGGCa-GUGGCCgCGU- -5' |
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| 25996 | 5' | -56.6 | NC_005340.1 | + | 28370 | 0.66 | 0.438409 |
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Target: 5'- --gCAgGGUGGGCCGUUuguCCGuCGCGa -3' miRNA: 3'- uaaGUgCCAUCUGGCAGu--GGCcGCGU- -5' |
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| 25996 | 5' | -56.6 | NC_005340.1 | + | 22408 | 0.69 | 0.303683 |
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Target: 5'- uGUUCACGGUuGACCGUaAUCGGUuuaGCu -3' miRNA: 3'- -UAAGUGCCAuCUGGCAgUGGCCG---CGu -5' |
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| 25996 | 5' | -56.6 | NC_005340.1 | + | 15226 | 0.69 | 0.296694 |
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Target: 5'- -aUCAaGGcucuccugacugcucAGGCCGUCACCGGCGUc -3' miRNA: 3'- uaAGUgCCa--------------UCUGGCAGUGGCCGCGu -5' |
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| 25996 | 5' | -56.6 | NC_005340.1 | + | 8201 | 0.69 | 0.280876 |
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Target: 5'- -cUCGCGcu-GGCCGUaACCGGCGCc -3' miRNA: 3'- uaAGUGCcauCUGGCAgUGGCCGCGu -5' |
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| 25996 | 5' | -56.6 | NC_005340.1 | + | 28297 | 1.06 | 0.000529 |
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Target: 5'- aAUUCACGGUAGACCGUCACCGGCGCAc -3' miRNA: 3'- -UAAGUGCCAUCUGGCAGUGGCCGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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