Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25997 | 3' | -52.6 | NC_005340.1 | + | 11379 | 0.66 | 0.664895 |
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Target: 5'- -cUUUAUC-CCGGCCcggaaagugaacccAUCAGGgcgGCGGc -3' miRNA: 3'- gaAAAUGGuGGCCGG--------------UAGUCCa--CGUC- -5' |
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| 25997 | 3' | -52.6 | NC_005340.1 | + | 14771 | 0.66 | 0.644884 |
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Target: 5'- -aUUUAgCGugguauauCCGGCCAgUCAGGcGCAGa -3' miRNA: 3'- gaAAAUgGU--------GGCCGGU-AGUCCaCGUC- -5' |
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| 25997 | 3' | -52.6 | NC_005340.1 | + | 2472 | 0.68 | 0.562715 |
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Target: 5'- ----cGCCGCCGGUgGuuUCGGGUGgCAa -3' miRNA: 3'- gaaaaUGGUGGCCGgU--AGUCCAC-GUc -5' |
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| 25997 | 3' | -52.6 | NC_005340.1 | + | 18915 | 0.68 | 0.53966 |
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Target: 5'- --aUUACCugGCCgauuguGGCCGUC-GGUGCGGc -3' miRNA: 3'- gaaAAUGG--UGG------CCGGUAGuCCACGUC- -5' |
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| 25997 | 3' | -52.6 | NC_005340.1 | + | 14844 | 0.7 | 0.440841 |
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Target: 5'- --aUUugUACCGGCCuGUCAGGUugaugGCAc -3' miRNA: 3'- gaaAAugGUGGCCGG-UAGUCCA-----CGUc -5' |
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| 25997 | 3' | -52.6 | NC_005340.1 | + | 20909 | 0.71 | 0.38096 |
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Target: 5'- uUUUUGCUgACCGGCUuugcuuUCGGGUGCu- -3' miRNA: 3'- gAAAAUGG-UGGCCGGu-----AGUCCACGuc -5' |
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| 25997 | 3' | -52.6 | NC_005340.1 | + | 20700 | 0.74 | 0.242748 |
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Target: 5'- --cUUACCGCUGGCCGUCAGc-GCAc -3' miRNA: 3'- gaaAAUGGUGGCCGGUAGUCcaCGUc -5' |
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| 25997 | 3' | -52.6 | NC_005340.1 | + | 7663 | 0.76 | 0.193577 |
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Target: 5'- ---gUAUUGCCGGUUAUCAGGUGguGg -3' miRNA: 3'- gaaaAUGGUGGCCGGUAGUCCACguC- -5' |
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| 25997 | 3' | -52.6 | NC_005340.1 | + | 28882 | 1.11 | 0.000609 |
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Target: 5'- aCUUUUACCACCGGCCAUCAGGUGCAGg -3' miRNA: 3'- -GAAAAUGGUGGCCGGUAGUCCACGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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