Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25998 | 5' | -50.8 | NC_005340.1 | + | 8613 | 0.66 | 0.73982 |
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Target: 5'- -cCGGcGCUGGCGCaGgcGGCGCGg- -3' miRNA: 3'- aaGUCuUGACCGUGaCaaCUGCGUac -5' |
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| 25998 | 5' | -50.8 | NC_005340.1 | + | 8173 | 0.66 | 0.728221 |
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Target: 5'- -aCAGucucgaaaAGCUGGuCACUGUcUGGCGCAc- -3' miRNA: 3'- aaGUC--------UUGACC-GUGACA-ACUGCGUac -5' |
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| 25998 | 5' | -50.8 | NC_005340.1 | + | 19610 | 0.67 | 0.655506 |
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Target: 5'- cUCAGAaaucgugggucauACUGGCACgc-UGGCGCGc- -3' miRNA: 3'- aAGUCU-------------UGACCGUGacaACUGCGUac -5' |
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| 25998 | 5' | -50.8 | NC_005340.1 | + | 26259 | 0.68 | 0.632504 |
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Target: 5'- gUUUGGAugUGGCAUgc-UGACGCAg- -3' miRNA: 3'- -AAGUCUugACCGUGacaACUGCGUac -5' |
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| 25998 | 5' | -50.8 | NC_005340.1 | + | 17906 | 0.68 | 0.620392 |
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Target: 5'- gUCAccGAGCUGGCGCgccUGUUuGCGCAg- -3' miRNA: 3'- aAGU--CUUGACCGUG---ACAAcUGCGUac -5' |
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| 25998 | 5' | -50.8 | NC_005340.1 | + | 17538 | 0.69 | 0.584179 |
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Target: 5'- cUCGGAgcGCUGGCGCUGUaccGAUGUu-- -3' miRNA: 3'- aAGUCU--UGACCGUGACAa--CUGCGuac -5' |
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| 25998 | 5' | -50.8 | NC_005340.1 | + | 108 | 0.7 | 0.513332 |
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Target: 5'- aUCGGGugUGGUugUGcgcgugUGGCGCGUc -3' miRNA: 3'- aAGUCUugACCGugACa-----ACUGCGUAc -5' |
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| 25998 | 5' | -50.8 | NC_005340.1 | + | 30564 | 1.06 | 0.001795 |
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Target: 5'- gUUCAGAACUGGCACUGUUGACGCAUGc -3' miRNA: 3'- -AAGUCUUGACCGUGACAACUGCGUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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