Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25999 | 5' | -56.5 | NC_005340.1 | + | 373 | 1.12 | 0.000226 |
Target: 5'- uGGAACGGCACGCGGUGAGCGUCCAUCa -3' miRNA: 3'- -CCUUGCCGUGCGCCACUCGCAGGUAG- -5' |
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25999 | 5' | -56.5 | NC_005340.1 | + | 7508 | 0.72 | 0.212355 |
Target: 5'- cGGAAaagugcUGGCAgGUGGUGAGC--CCAUCa -3' miRNA: 3'- -CCUU------GCCGUgCGCCACUCGcaGGUAG- -5' |
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25999 | 5' | -56.5 | NC_005340.1 | + | 6590 | 0.68 | 0.357248 |
Target: 5'- aGAcCGGCAgcaaaGCGc-GGGCGUCCAUCa -3' miRNA: 3'- cCUuGCCGUg----CGCcaCUCGCAGGUAG- -5' |
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25999 | 5' | -56.5 | NC_005340.1 | + | 6661 | 0.66 | 0.507421 |
Target: 5'- cGGGCGGCACGCcGGUcaaagcggguuuuGAcccugacagcgaccgGCGUCCAc- -3' miRNA: 3'- cCUUGCCGUGCG-CCA-------------CU---------------CGCAGGUag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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