Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 260 | 3' | -53.5 | AC_000008.1 | + | 13104 | 0.66 | 0.683238 |
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Target: 5'- aGGCGCAuGUGGACGAGCauaCUUUCc- -3' miRNA: 3'- -CUGCGUuUAUCUGCUCGga-GGGAGca -5' |
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| 260 | 3' | -53.5 | AC_000008.1 | + | 8999 | 0.67 | 0.637213 |
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Target: 5'- aGACGCGGcuGUAGACcacGCCcCCUUCGg -3' miRNA: 3'- -CUGCGUU--UAUCUGcu-CGGaGGGAGCa -5' |
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| 260 | 3' | -53.5 | AC_000008.1 | + | 29693 | 0.67 | 0.625665 |
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Target: 5'- uGAC-CAGAUGGAUGcGGCCaCCCUCa- -3' miRNA: 3'- -CUGcGUUUAUCUGC-UCGGaGGGAGca -5' |
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| 260 | 3' | -53.5 | AC_000008.1 | + | 23819 | 0.72 | 0.335001 |
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Target: 5'- aGCGCGucuuguGAUGAGUCUUCCUCGUc -3' miRNA: 3'- cUGCGUuuau--CUGCUCGGAGGGAGCA- -5' |
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| 260 | 3' | -53.5 | AC_000008.1 | + | 8968 | 0.77 | 0.160365 |
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Target: 5'- -cUGCGAGaAGGCGuuaGGCCUCCCUCGUu -3' miRNA: 3'- cuGCGUUUaUCUGC---UCGGAGGGAGCA- -5' |
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| 260 | 3' | -53.5 | AC_000008.1 | + | 18409 | 1.08 | 0.001015 |
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Target: 5'- uGACGCAAAUAGACGAGCCUCCCUCGUa -3' miRNA: 3'- -CUGCGUUUAUCUGCUCGGAGGGAGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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