Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26008 | 3' | -54.9 | NC_005340.1 | + | 4710 | 0.66 | 0.533771 |
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Target: 5'- aGCGGUGGUgGuGGCGAugagcuGCUGAc-- -3' miRNA: 3'- cCGCCGCCGaCuCCGUUu-----CGACUuuc -5' |
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| 26008 | 3' | -54.9 | NC_005340.1 | + | 16193 | 0.66 | 0.522571 |
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Target: 5'- cGGCGGCGGUaUGAauGGCGcGGCa----- -3' miRNA: 3'- -CCGCCGCCG-ACU--CCGUuUCGacuuuc -5' |
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| 26008 | 3' | -54.9 | NC_005340.1 | + | 5317 | 0.66 | 0.511463 |
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Target: 5'- aGGauGUGGCUGuAGGCu-GGCUGcaAAAGu -3' miRNA: 3'- -CCgcCGCCGAC-UCCGuuUCGAC--UUUC- -5' |
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| 26008 | 3' | -54.9 | NC_005340.1 | + | 20274 | 0.73 | 0.211014 |
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Target: 5'- gGGCGGUGcugacGCUGuGGCuuGGCUGgcAGg -3' miRNA: 3'- -CCGCCGC-----CGACuCCGuuUCGACuuUC- -5' |
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| 26008 | 3' | -54.9 | NC_005340.1 | + | 3372 | 0.74 | 0.163339 |
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Target: 5'- aGCGaugaGGCUGucGCAAAGCUGGAAGa -3' miRNA: 3'- cCGCcg--CCGACucCGUUUCGACUUUC- -5' |
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| 26008 | 3' | -54.9 | NC_005340.1 | + | 9172 | 0.8 | 0.068838 |
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Target: 5'- aGCGGUGGCUcaGGGGCAuAGCUGAc-- -3' miRNA: 3'- cCGCCGCCGA--CUCCGUuUCGACUuuc -5' |
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| 26008 | 3' | -54.9 | NC_005340.1 | + | 11410 | 1.1 | 0.000376 |
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Target: 5'- gGGCGGCGGCUGAGGCAAAGCUGAAAGc -3' miRNA: 3'- -CCGCCGCCGACUCCGUUUCGACUUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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