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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26011 | 5' | -56.9 | NC_005340.1 | + | 7329 | 0.67 | 0.3404 |
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Target: 5'- -cUGCAGGCuaaucCCGGCcuguCUGA-GCUGg -3' miRNA: 3'- aaACGUCCGua---GGCCGu---GGCUaCGAC- -5' |
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| 26011 | 5' | -56.9 | NC_005340.1 | + | 3978 | 0.67 | 0.3318 |
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Target: 5'- --cGCAGGUAUCacccuCGaCGCCGAUGCg- -3' miRNA: 3'- aaaCGUCCGUAG-----GCcGUGGCUACGac -5' |
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| 26011 | 5' | -56.9 | NC_005340.1 | + | 3694 | 0.67 | 0.323361 |
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Target: 5'- --gGCAGGCuuuUCCGuGCcacagauuGCCGAaaUGCUGc -3' miRNA: 3'- aaaCGUCCGu--AGGC-CG--------UGGCU--ACGAC- -5' |
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| 26011 | 5' | -56.9 | NC_005340.1 | + | 5510 | 0.68 | 0.315083 |
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Target: 5'- gUUGguGGCAUCCaucaccaguGCAUCGAUGUUc -3' miRNA: 3'- aAACguCCGUAGGc--------CGUGGCUACGAc -5' |
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| 26011 | 5' | -56.9 | NC_005340.1 | + | 16297 | 0.69 | 0.268785 |
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Target: 5'- --cGCAGuacGCcgCCccgGGCGCUGAUGCUGu -3' miRNA: 3'- aaaCGUC---CGuaGG---CCGUGGCUACGAC- -5' |
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| 26011 | 5' | -56.9 | NC_005340.1 | + | 15611 | 1.06 | 0.000349 |
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Target: 5'- aUUUGCAGGCAUCCGGCACCGAUGCUGa -3' miRNA: 3'- -AAACGUCCGUAGGCCGUGGCUACGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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