Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26014 | 3' | -57.1 | NC_005340.1 | + | 9227 | 0.66 | 0.443275 |
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Target: 5'- aGGAGCGCCuuACCGGUCUGAUugCCagccugucaccggcgGCGCGu -3' miRNA: 3'- -UUUUGUGG--UGGUCAGGCUG--GG---------------CGCGC- -5' |
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| 26014 | 3' | -57.1 | NC_005340.1 | + | 6631 | 0.66 | 0.416507 |
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Target: 5'- ----aACCGCCGGUuaacugaacgcuccCCGAgCCGgGCGg -3' miRNA: 3'- uuuugUGGUGGUCA--------------GGCUgGGCgCGC- -5' |
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| 26014 | 3' | -57.1 | NC_005340.1 | + | 3982 | 0.67 | 0.381478 |
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Target: 5'- gAAAugGCCgaACCua--UGACCCGCGCGu -3' miRNA: 3'- -UUUugUGG--UGGucagGCUGGGCGCGC- -5' |
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| 26014 | 3' | -57.1 | NC_005340.1 | + | 17374 | 0.69 | 0.274765 |
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Target: 5'- cAAGCGCCugACCAGUUaCGGCCaguuGCGCGc -3' miRNA: 3'- uUUUGUGG--UGGUCAG-GCUGGg---CGCGC- -5' |
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| 26014 | 3' | -57.1 | NC_005340.1 | + | 9800 | 0.7 | 0.260642 |
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Target: 5'- -cGAUACCGCgCAGgggCUGugCCGCGUa -3' miRNA: 3'- uuUUGUGGUG-GUCa--GGCugGGCGCGc -5' |
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| 26014 | 3' | -57.1 | NC_005340.1 | + | 9934 | 0.73 | 0.145838 |
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Target: 5'- gGGAGC-CCACCAuGUCCGcgaacgACCgGCGCGg -3' miRNA: 3'- -UUUUGuGGUGGU-CAGGC------UGGgCGCGC- -5' |
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| 26014 | 3' | -57.1 | NC_005340.1 | + | 6538 | 0.73 | 0.141724 |
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Target: 5'- gGAGGCACCACCAcGUaaaaCGACuaaaCCGCGCa -3' miRNA: 3'- -UUUUGUGGUGGU-CAg---GCUG----GGCGCGc -5' |
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| 26014 | 3' | -57.1 | NC_005340.1 | + | 16867 | 0.76 | 0.094332 |
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Target: 5'- ---cCGCCGCCAGaCUGACgCCGCGCa -3' miRNA: 3'- uuuuGUGGUGGUCaGGCUG-GGCGCGc -5' |
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| 26014 | 3' | -57.1 | NC_005340.1 | + | 17386 | 1.05 | 0.00053 |
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Target: 5'- aAAAACACCACCAGUCCGACCCGCGCGc -3' miRNA: 3'- -UUUUGUGGUGGUCAGGCUGGGCGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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