Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26015 | 3' | -56.2 | NC_005340.1 | + | 24384 | 0.67 | 0.435738 |
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Target: 5'- cCACgGGCGGcGGCGaaguCUUUUAC--UGACa -3' miRNA: 3'- -GUGgCCGCC-CCGC----GAGAAUGuaACUG- -5' |
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| 26015 | 3' | -56.2 | NC_005340.1 | + | 11976 | 0.67 | 0.409976 |
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Target: 5'- uGCCgGGCGGGuCGCUgCUuucccaccauuccguUACAUUGAUg -3' miRNA: 3'- gUGG-CCGCCCcGCGA-GA---------------AUGUAACUG- -5' |
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| 26015 | 3' | -56.2 | NC_005340.1 | + | 2798 | 0.67 | 0.405131 |
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Target: 5'- uCGCCGGUGGGcuucuuuGCGCUgaCggACAgUGGCg -3' miRNA: 3'- -GUGGCCGCCC-------CGCGA--GaaUGUaACUG- -5' |
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| 26015 | 3' | -56.2 | NC_005340.1 | + | 29960 | 0.68 | 0.377757 |
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Target: 5'- uGCCGGCGGGGUugaacaacgaGCgcagCgagGCGUUaGACu -3' miRNA: 3'- gUGGCCGCCCCG----------CGa---Gaa-UGUAA-CUG- -5' |
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| 26015 | 3' | -56.2 | NC_005340.1 | + | 19409 | 1.08 | 0.000395 |
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Target: 5'- gCACCGGCGGGGCGCUCUUACAUUGACc -3' miRNA: 3'- -GUGGCCGCCCCGCGAGAAUGUAACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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