Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26016 | 3' | -47.6 | NC_005340.1 | + | 19951 | 0.67 | 0.890708 |
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Target: 5'- gCGCCAUCGCUGA--AAAAcucGGCg -3' miRNA: 3'- gGCGGUAGCGAUUauUUUUuccCCGa -5' |
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| 26016 | 3' | -47.6 | NC_005340.1 | + | 10044 | 0.68 | 0.856205 |
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Target: 5'- uCCGCCGagGCUGAc------GGGGCa -3' miRNA: 3'- -GGCGGUagCGAUUauuuuuuCCCCGa -5' |
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| 26016 | 3' | -47.6 | NC_005340.1 | + | 8820 | 0.69 | 0.827292 |
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Target: 5'- gCGCCuguUCGCUGAUAAc----GGGCa -3' miRNA: 3'- gGCGGu--AGCGAUUAUUuuuucCCCGa -5' |
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| 26016 | 3' | -47.6 | NC_005340.1 | + | 14466 | 0.72 | 0.668849 |
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Target: 5'- aCCGUCG-CGCUGAUAuuucAGGcGGCUg -3' miRNA: 3'- -GGCGGUaGCGAUUAUuuuuUCC-CCGA- -5' |
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| 26016 | 3' | -47.6 | NC_005340.1 | + | 15038 | 0.72 | 0.656724 |
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Target: 5'- gCGCCAUUGCgaaagccGGUAAAAAAGGcacgcuGGCUg -3' miRNA: 3'- gGCGGUAGCGa------UUAUUUUUUCC------CCGA- -5' |
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| 26016 | 3' | -47.6 | NC_005340.1 | + | 13284 | 0.74 | 0.536095 |
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Target: 5'- cUCGCUggCGCUGugGUGGAGGuuauGGGGGCUg -3' miRNA: 3'- -GGCGGuaGCGAU--UAUUUUU----UCCCCGA- -5' |
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| 26016 | 3' | -47.6 | NC_005340.1 | + | 28234 | 0.78 | 0.309938 |
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Target: 5'- gCCGCCcaUGCUGAUAcAGcGGGGGCUg -3' miRNA: 3'- -GGCGGuaGCGAUUAUuUUuUCCCCGA- -5' |
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| 26016 | 3' | -47.6 | NC_005340.1 | + | 21598 | 1.12 | 0.001991 |
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Target: 5'- gCCGCCAUCGCUAAUAAAAAAGGGGCUg -3' miRNA: 3'- -GGCGGUAGCGAUUAUUUUUUCCCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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