Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26019 | 3' | -55 | NC_005342.2 | + | 31572 | 0.65 | 0.700155 |
Target: 5'- gGUUGUuUGCcUGCaucuGCUGAAUCGCGGCa -3' miRNA: 3'- -UAGCAcACGaGCG----CGGCUUAGUGCUG- -5' |
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26019 | 3' | -55 | NC_005342.2 | + | 4134 | 0.66 | 0.678018 |
Target: 5'- uUCGUGUacagcgcgccgcGCUCGUGCUGca--GCGGCg -3' miRNA: 3'- uAGCACA------------CGAGCGCGGCuuagUGCUG- -5' |
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26019 | 3' | -55 | NC_005342.2 | + | 34993 | 0.66 | 0.674679 |
Target: 5'- cGUCGauuUGUcGaugCGCGCCGAgcugaucaaccgcuAUCGCGACa -3' miRNA: 3'- -UAGC---ACA-Cga-GCGCGGCU--------------UAGUGCUG- -5' |
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26019 | 3' | -55 | NC_005342.2 | + | 36097 | 0.66 | 0.666875 |
Target: 5'- -aCGacaagGUGCUCGCGCCc-GUCuCGGCc -3' miRNA: 3'- uaGCa----CACGAGCGCGGcuUAGuGCUG- -5' |
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26019 | 3' | -55 | NC_005342.2 | + | 19193 | 0.66 | 0.654579 |
Target: 5'- gAUCGUgggcggcgcaGUGCUCGCGCuCGucAUCgugcuggccgcacGCGACg -3' miRNA: 3'- -UAGCA----------CACGAGCGCG-GCu-UAG-------------UGCUG- -5' |
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26019 | 3' | -55 | NC_005342.2 | + | 45917 | 0.66 | 0.6445 |
Target: 5'- -gCGcGU-CUCGCGCCGGAUCAgCGcCa -3' miRNA: 3'- uaGCaCAcGAGCGCGGCUUAGU-GCuG- -5' |
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26019 | 3' | -55 | NC_005342.2 | + | 2695 | 0.66 | 0.6445 |
Target: 5'- cGUCGgcgagGuUGUUCGUGCCc-GUCACGGCc -3' miRNA: 3'- -UAGCa----C-ACGAGCGCGGcuUAGUGCUG- -5' |
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26019 | 3' | -55 | NC_005342.2 | + | 12148 | 0.67 | 0.610875 |
Target: 5'- -aCGUGgcugGCgcgcaGCGCCGcGUCACGGg -3' miRNA: 3'- uaGCACa---CGag---CGCGGCuUAGUGCUg -5' |
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26019 | 3' | -55 | NC_005342.2 | + | 9309 | 0.67 | 0.610875 |
Target: 5'- cUCGgcgcuuucGUGUUCGuCGUCGGcgCGCGGCg -3' miRNA: 3'- uAGCa-------CACGAGC-GCGGCUuaGUGCUG- -5' |
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26019 | 3' | -55 | NC_005342.2 | + | 19913 | 0.68 | 0.56635 |
Target: 5'- ----cGUGCUCaCGCCGA-UCACGAg -3' miRNA: 3'- uagcaCACGAGcGCGGCUuAGUGCUg -5' |
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26019 | 3' | -55 | NC_005342.2 | + | 30120 | 0.68 | 0.555335 |
Target: 5'- uUCGaacUG-GCaCGCGCCGAuggCGCGACg -3' miRNA: 3'- uAGC---ACaCGaGCGCGGCUua-GUGCUG- -5' |
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26019 | 3' | -55 | NC_005342.2 | + | 28381 | 0.68 | 0.552043 |
Target: 5'- gAUCGcgcgcgauuccccuUGUGC-CGCGCCGAccgUGCGACc -3' miRNA: 3'- -UAGC--------------ACACGaGCGCGGCUua-GUGCUG- -5' |
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26019 | 3' | -55 | NC_005342.2 | + | 28726 | 0.68 | 0.544384 |
Target: 5'- -gCGUGUGCgUCGCGUgCGGccagCACGAUg -3' miRNA: 3'- uaGCACACG-AGCGCG-GCUua--GUGCUG- -5' |
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26019 | 3' | -55 | NC_005342.2 | + | 8932 | 0.68 | 0.544384 |
Target: 5'- cAUCGcGUGCgCGgGCUGGAUCguGCGGCc -3' miRNA: 3'- -UAGCaCACGaGCgCGGCUUAG--UGCUG- -5' |
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26019 | 3' | -55 | NC_005342.2 | + | 18196 | 0.68 | 0.527013 |
Target: 5'- cGUCGUGcGCgcggcaucgcaauaCGCGCCGGAaaUCuACGACg -3' miRNA: 3'- -UAGCACaCGa-------------GCGCGGCUU--AG-UGCUG- -5' |
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26019 | 3' | -55 | NC_005342.2 | + | 1102 | 0.69 | 0.511986 |
Target: 5'- aGUCG-GUGC-CGUGCUGcg-CGCGACg -3' miRNA: 3'- -UAGCaCACGaGCGCGGCuuaGUGCUG- -5' |
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26019 | 3' | -55 | NC_005342.2 | + | 569 | 0.69 | 0.484569 |
Target: 5'- uUCaUGUGCUCGCGCgGAucgaacgugccgcccAUCucgGCGACg -3' miRNA: 3'- uAGcACACGAGCGCGgCU---------------UAG---UGCUG- -5' |
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26019 | 3' | -55 | NC_005342.2 | + | 47821 | 0.7 | 0.449819 |
Target: 5'- uGUCGUGcUGCUCgGUGCCGAcgaggCGCaGGCg -3' miRNA: 3'- -UAGCAC-ACGAG-CGCGGCUua---GUG-CUG- -5' |
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26019 | 3' | -55 | NC_005342.2 | + | 21096 | 0.7 | 0.448818 |
Target: 5'- cAUCGg--GCUCGCaguguuuGCCGAG-CGCGACg -3' miRNA: 3'- -UAGCacaCGAGCG-------CGGCUUaGUGCUG- -5' |
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26019 | 3' | -55 | NC_005342.2 | + | 34323 | 0.71 | 0.391118 |
Target: 5'- cUCGUGcUGCUCGCGCagcugccCGAGUaccgCGCGAUg -3' miRNA: 3'- uAGCAC-ACGAGCGCG-------GCUUA----GUGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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