Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26019 | 3' | -55 | NC_005342.2 | + | 21096 | 0.7 | 0.448818 |
Target: 5'- cAUCGg--GCUCGCaguguuuGCCGAG-CGCGACg -3' miRNA: 3'- -UAGCacaCGAGCG-------CGGCUUaGUGCUG- -5' |
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26019 | 3' | -55 | NC_005342.2 | + | 34323 | 0.71 | 0.391118 |
Target: 5'- cUCGUGcUGCUCGCGCagcugccCGAGUaccgCGCGAUg -3' miRNA: 3'- uAGCAC-ACGAGCGCG-------GCUUA----GUGCUG- -5' |
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26019 | 3' | -55 | NC_005342.2 | + | 28869 | 0.76 | 0.189968 |
Target: 5'- aAUCGUGacggucacacggGC-CGCGCCGAGUgGCGACg -3' miRNA: 3'- -UAGCACa-----------CGaGCGCGGCUUAgUGCUG- -5' |
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26019 | 3' | -55 | NC_005342.2 | + | 34993 | 0.66 | 0.674679 |
Target: 5'- cGUCGauuUGUcGaugCGCGCCGAgcugaucaaccgcuAUCGCGACa -3' miRNA: 3'- -UAGC---ACA-Cga-GCGCGGCU--------------UAGUGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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