Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26020 | 3' | -53.7 | NC_005342.2 | + | 24773 | 0.66 | 0.773303 |
Target: 5'- uAUCAcgaUUCCGCAaaacGGCGcacCGACCGacgUGACg -3' miRNA: 3'- -UAGU---AAGGCGU----CUGCa--GCUGGC---ACUG- -5' |
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26020 | 3' | -53.7 | NC_005342.2 | + | 33516 | 0.66 | 0.773303 |
Target: 5'- gAUCGaugCUGCGGACG-CGAuauuacCCGUGAUg -3' miRNA: 3'- -UAGUaa-GGCGUCUGCaGCU------GGCACUG- -5' |
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26020 | 3' | -53.7 | NC_005342.2 | + | 44367 | 0.66 | 0.762922 |
Target: 5'- -aCGgcCCGCAGGCGccCGACUG-GGCg -3' miRNA: 3'- uaGUaaGGCGUCUGCa-GCUGGCaCUG- -5' |
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26020 | 3' | -53.7 | NC_005342.2 | + | 30424 | 0.66 | 0.762922 |
Target: 5'- cUUcgUCCuggaaGCuGGCGagcUCGGCCGUGGCg -3' miRNA: 3'- uAGuaAGG-----CGuCUGC---AGCUGGCACUG- -5' |
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26020 | 3' | -53.7 | NC_005342.2 | + | 42921 | 0.66 | 0.7524 |
Target: 5'- uUCAgcgCCGCGccGACGUCGGCCa---- -3' miRNA: 3'- uAGUaa-GGCGU--CUGCAGCUGGcacug -5' |
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26020 | 3' | -53.7 | NC_005342.2 | + | 19215 | 0.67 | 0.688116 |
Target: 5'- -gCGUUCCGCAcGGCGcUCGAgcaauacgggcuggaCGUGACg -3' miRNA: 3'- uaGUAAGGCGU-CUGC-AGCUg--------------GCACUG- -5' |
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26020 | 3' | -53.7 | NC_005342.2 | + | 37312 | 0.67 | 0.687002 |
Target: 5'- -gCGUgcaCCgGCcGGCG-CGACCGUGACg -3' miRNA: 3'- uaGUAa--GG-CGuCUGCaGCUGGCACUG- -5' |
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26020 | 3' | -53.7 | NC_005342.2 | + | 8660 | 0.67 | 0.675839 |
Target: 5'- -gCGUUCaGCAcGGCGaacgugcCGACCGUGACg -3' miRNA: 3'- uaGUAAGgCGU-CUGCa------GCUGGCACUG- -5' |
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26020 | 3' | -53.7 | NC_005342.2 | + | 45561 | 0.68 | 0.653394 |
Target: 5'- gGUCGgugCUGCucGAUGUCGAUCGUGuCg -3' miRNA: 3'- -UAGUaa-GGCGu-CUGCAGCUGGCACuG- -5' |
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26020 | 3' | -53.7 | NC_005342.2 | + | 45943 | 0.7 | 0.527413 |
Target: 5'- ------gCGCGGGCGUCGaugugaucgacgcgGCCGUGACg -3' miRNA: 3'- uaguaagGCGUCUGCAGC--------------UGGCACUG- -5' |
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26020 | 3' | -53.7 | NC_005342.2 | + | 16528 | 0.74 | 0.296511 |
Target: 5'- cGUCGagaUUCCGCAGaucGCGUCGAgCGUcGACg -3' miRNA: 3'- -UAGU---AAGGCGUC---UGCAGCUgGCA-CUG- -5' |
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26020 | 3' | -53.7 | NC_005342.2 | + | 47689 | 1.07 | 0.001702 |
Target: 5'- cAUCAUUCCGCAGACGUCGACCGUGACc -3' miRNA: 3'- -UAGUAAGGCGUCUGCAGCUGGCACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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