Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26027 | 5' | -55 | NC_005342.2 | + | 30679 | 0.66 | 0.704853 |
Target: 5'- cGGccGUCAGGcgCGacuccauaaagcccuGCUGCUGCGGgCUg -3' miRNA: 3'- aCCa-UAGUCCa-GC---------------UGACGAUGCCgGG- -5' |
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26027 | 5' | -55 | NC_005342.2 | + | 39283 | 0.66 | 0.700494 |
Target: 5'- cGGcGUCAcGGUCGGCacguucgccgUGCUgaacgccgACGGCaCCg -3' miRNA: 3'- aCCaUAGU-CCAGCUG----------ACGA--------UGCCG-GG- -5' |
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26027 | 5' | -55 | NC_005342.2 | + | 29060 | 0.66 | 0.682955 |
Target: 5'- gGGUGUacCAGccgcgCGGCUGCUuaacacgcaccucgaGCGGCUCg -3' miRNA: 3'- aCCAUA--GUCca---GCUGACGA---------------UGCCGGG- -5' |
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26027 | 5' | -55 | NC_005342.2 | + | 17934 | 0.66 | 0.678549 |
Target: 5'- gGGUGgcgucgCAGGUgCGACg---GCGGCCa -3' miRNA: 3'- aCCAUa-----GUCCA-GCUGacgaUGCCGGg -5' |
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26027 | 5' | -55 | NC_005342.2 | + | 13412 | 0.67 | 0.623092 |
Target: 5'- ---cGUCAGGgauUCGACUGCUgGCgGGCaCCu -3' miRNA: 3'- accaUAGUCC---AGCUGACGA-UG-CCG-GG- -5' |
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26027 | 5' | -55 | NC_005342.2 | + | 41350 | 0.67 | 0.623092 |
Target: 5'- cGGcagauUGUCGGG-CG-CggGCUGCGGCCg -3' miRNA: 3'- aCC-----AUAGUCCaGCuGa-CGAUGCCGGg -5' |
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26027 | 5' | -55 | NC_005342.2 | + | 33772 | 0.68 | 0.603109 |
Target: 5'- aUGG-GUCuGGUCGAacaauugcgucaUGCUgccgcacccggacgaGCGGCCCg -3' miRNA: 3'- -ACCaUAGuCCAGCUg-----------ACGA---------------UGCCGGG- -5' |
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26027 | 5' | -55 | NC_005342.2 | + | 36556 | 0.68 | 0.600893 |
Target: 5'- cGGUcgaAGG-CGACgUGCUGCGGCa- -3' miRNA: 3'- aCCAuagUCCaGCUG-ACGAUGCCGgg -5' |
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26027 | 5' | -55 | NC_005342.2 | + | 31958 | 0.68 | 0.556893 |
Target: 5'- cGGaUAUC-GGUUGGCaGCaGCGGCCg -3' miRNA: 3'- aCC-AUAGuCCAGCUGaCGaUGCCGGg -5' |
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26027 | 5' | -55 | NC_005342.2 | + | 22282 | 0.68 | 0.546025 |
Target: 5'- cGG-AUCAGGUCGcCgccuucGCgACGGCCg -3' miRNA: 3'- aCCaUAGUCCAGCuGa-----CGaUGCCGGg -5' |
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26027 | 5' | -55 | NC_005342.2 | + | 15761 | 0.69 | 0.535227 |
Target: 5'- cUGGUGUUguccGGUUacaUGCUGCGGCUCg -3' miRNA: 3'- -ACCAUAGu---CCAGcugACGAUGCCGGG- -5' |
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26027 | 5' | -55 | NC_005342.2 | + | 40357 | 0.69 | 0.503314 |
Target: 5'- cUGGUAUCAGGcCGAa----GCGGCCg -3' miRNA: 3'- -ACCAUAGUCCaGCUgacgaUGCCGGg -5' |
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26027 | 5' | -55 | NC_005342.2 | + | 13784 | 0.72 | 0.350468 |
Target: 5'- aUGGUAuUCAGGUCuaugaUGCggGCGGCCUc -3' miRNA: 3'- -ACCAU-AGUCCAGcug--ACGa-UGCCGGG- -5' |
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26027 | 5' | -55 | NC_005342.2 | + | 22185 | 0.72 | 0.350468 |
Target: 5'- cGGcggCGGuUCGGCgGCUGCGGCCUg -3' miRNA: 3'- aCCauaGUCcAGCUGaCGAUGCCGGG- -5' |
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26027 | 5' | -55 | NC_005342.2 | + | 44347 | 1.13 | 0.000511 |
Target: 5'- cUGGUAUCAGGUCGACUGCUACGGCCCg -3' miRNA: 3'- -ACCAUAGUCCAGCUGACGAUGCCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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