Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26034 | 5' | -46.1 | NC_005342.2 | + | 45240 | 0.66 | 0.995109 |
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Target: 5'- cGGggCGCCCCGcAUUCGag-CGguGGu -3' miRNA: 3'- -CCaaGCGGGGCuUAAGUaaaGUguUC- -5' |
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| 26034 | 5' | -46.1 | NC_005342.2 | + | 2952 | 0.66 | 0.993193 |
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Target: 5'- ---aCGCCUCGAGcUUCGggacgucgUUCGCGAGc -3' miRNA: 3'- ccaaGCGGGGCUU-AAGUa-------AAGUGUUC- -5' |
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| 26034 | 5' | -46.1 | NC_005342.2 | + | 27921 | 0.68 | 0.972928 |
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Target: 5'- cGUUCGCCUCGA-------UCACAAGc -3' miRNA: 3'- cCAAGCGGGGCUuaaguaaAGUGUUC- -5' |
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| 26034 | 5' | -46.1 | NC_005342.2 | + | 2088 | 0.71 | 0.914927 |
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Target: 5'- --aUCGCCUCGAAUUCGg--CGCGc- -3' miRNA: 3'- ccaAGCGGGGCUUAAGUaaaGUGUuc -5' |
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| 26034 | 5' | -46.1 | NC_005342.2 | + | 5710 | 0.76 | 0.692283 |
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Target: 5'- gGGUaCGCCCCGAAcgUUAg--CACAAGu -3' miRNA: 3'- -CCAaGCGGGGCUUa-AGUaaaGUGUUC- -5' |
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| 26034 | 5' | -46.1 | NC_005342.2 | + | 42273 | 1.13 | 0.005042 |
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Target: 5'- aGGUUCGCCCCGAAUUCAUUUCACAAGu -3' miRNA: 3'- -CCAAGCGGGGCUUAAGUAAAGUGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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