Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26036 | 3' | -53.5 | NC_005342.2 | + | 21453 | 0.66 | 0.77819 |
Target: 5'- gCCGGaCUucaucgacGGAaAUGGCGUGCGgcacgCGACg -3' miRNA: 3'- -GGCCaGA--------CCUaUACCGCGUGUa----GCUG- -5' |
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26036 | 3' | -53.5 | NC_005342.2 | + | 31977 | 0.66 | 0.77819 |
Target: 5'- gCGGccgaaagccuUCcGGA--UGGCGCGgGUCGGCg -3' miRNA: 3'- gGCC----------AGaCCUauACCGCGUgUAGCUG- -5' |
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26036 | 3' | -53.5 | NC_005342.2 | + | 40572 | 0.66 | 0.77819 |
Target: 5'- uCUGGUCgccGGcgAUGGuCGCGCGacUCGGu -3' miRNA: 3'- -GGCCAGa--CCuaUACC-GCGUGU--AGCUg -5' |
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26036 | 3' | -53.5 | NC_005342.2 | + | 1566 | 0.66 | 0.767948 |
Target: 5'- uUCGGUaUUGGucgguUGGgGCAUGUCGAUa -3' miRNA: 3'- -GGCCA-GACCuau--ACCgCGUGUAGCUG- -5' |
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26036 | 3' | -53.5 | NC_005342.2 | + | 13811 | 0.67 | 0.747039 |
Target: 5'- aCGGUUcGGcgAcgGGCGCcGCGUCGAg -3' miRNA: 3'- gGCCAGaCCuaUa-CCGCG-UGUAGCUg -5' |
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26036 | 3' | -53.5 | NC_005342.2 | + | 28828 | 0.67 | 0.719152 |
Target: 5'- uCCGGUCagUGGAUAgucGGCggccgaucauaguccGCcaucGCGUCGGCg -3' miRNA: 3'- -GGCCAG--ACCUAUa--CCG---------------CG----UGUAGCUG- -5' |
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26036 | 3' | -53.5 | NC_005342.2 | + | 29521 | 0.68 | 0.66958 |
Target: 5'- aCGGUCaccgggccguugaUGGcgAUcGGCGCGCcgccgGUCGACu -3' miRNA: 3'- gGCCAG-------------ACCuaUA-CCGCGUG-----UAGCUG- -5' |
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26036 | 3' | -53.5 | NC_005342.2 | + | 41381 | 0.69 | 0.625979 |
Target: 5'- gCGGcgCUGGGc--GGCGCGCcgUGGCg -3' miRNA: 3'- gGCCa-GACCUauaCCGCGUGuaGCUG- -5' |
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26036 | 3' | -53.5 | NC_005342.2 | + | 12326 | 0.7 | 0.559256 |
Target: 5'- uUCGGcCUGuucgccGAUGUGGCGUAUAaCGGCg -3' miRNA: 3'- -GGCCaGAC------CUAUACCGCGUGUaGCUG- -5' |
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26036 | 3' | -53.5 | NC_005342.2 | + | 17911 | 0.7 | 0.537407 |
Target: 5'- gCCGGUgcggccgcgcgcCUGGcggGUGGCGuCGCAggugCGACg -3' miRNA: 3'- -GGCCA------------GACCua-UACCGC-GUGUa---GCUG- -5' |
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26036 | 3' | -53.5 | NC_005342.2 | + | 7015 | 0.71 | 0.505216 |
Target: 5'- gUCGGUUUGaacUGUGcGCGCAgAUCGGCg -3' miRNA: 3'- -GGCCAGACcu-AUAC-CGCGUgUAGCUG- -5' |
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26036 | 3' | -53.5 | NC_005342.2 | + | 10569 | 0.72 | 0.414351 |
Target: 5'- gCCGGcCgacgagcGGGUcgGGCGCGCGcUCGAUg -3' miRNA: 3'- -GGCCaGa------CCUAuaCCGCGUGU-AGCUG- -5' |
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26036 | 3' | -53.5 | NC_005342.2 | + | 17439 | 0.74 | 0.318055 |
Target: 5'- cCCGGgcgCcGGAUGUGGCGCAacgCGAa -3' miRNA: 3'- -GGCCa--GaCCUAUACCGCGUguaGCUg -5' |
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26036 | 3' | -53.5 | NC_005342.2 | + | 7928 | 0.76 | 0.273059 |
Target: 5'- cCCGGguuuUCgaaaGAUcgGGCGUACAUCGACa -3' miRNA: 3'- -GGCC----AGac--CUAuaCCGCGUGUAGCUG- -5' |
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26036 | 3' | -53.5 | NC_005342.2 | + | 40893 | 1.08 | 0.001646 |
Target: 5'- gCCGGUCUGGAUAUGGCGCACAUCGAUu -3' miRNA: 3'- -GGCCAGACCUAUACCGCGUGUAGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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