Results 1 - 20 of 42 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26037 | 3' | -55.7 | NC_005342.2 | + | 38550 | 0.66 | 0.621045 |
Target: 5'- gGCgaaccggacgGCGuGCGCAGccCGCAGGGCAg- -3' miRNA: 3'- aCGa---------CGUcUGCGUUa-GCGUCCCGUag -5' |
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26037 | 3' | -55.7 | NC_005342.2 | + | 26401 | 0.66 | 0.621045 |
Target: 5'- gGCUGCAG-CGCGGaaaGUGGgauaGGCGUCa -3' miRNA: 3'- aCGACGUCuGCGUUag-CGUC----CCGUAG- -5' |
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26037 | 3' | -55.7 | NC_005342.2 | + | 7065 | 0.66 | 0.599595 |
Target: 5'- gUGCgccauauccagaccgGCAGcAUGCAAUCGCAGcGCAa- -3' miRNA: 3'- -ACGa--------------CGUC-UGCGUUAGCGUCcCGUag -5' |
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26037 | 3' | -55.7 | NC_005342.2 | + | 45015 | 0.66 | 0.598469 |
Target: 5'- aGCUcgAGGCGU--UCGagaAGGGCAUCg -3' miRNA: 3'- aCGAcgUCUGCGuuAGCg--UCCCGUAG- -5' |
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26037 | 3' | -55.7 | NC_005342.2 | + | 20954 | 0.66 | 0.587221 |
Target: 5'- cGggGCGGACGCAAaCGCGcgcggcgcGGGCGa- -3' miRNA: 3'- aCgaCGUCUGCGUUaGCGU--------CCCGUag -5' |
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26037 | 3' | -55.7 | NC_005342.2 | + | 17332 | 0.66 | 0.587221 |
Target: 5'- cGCUGUAuaGCGCA--CGCAGcGGCGUg -3' miRNA: 3'- aCGACGUc-UGCGUuaGCGUC-CCGUAg -5' |
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26037 | 3' | -55.7 | NC_005342.2 | + | 11086 | 0.66 | 0.587221 |
Target: 5'- cGCUGCGGgaugccgcACGUGAUCGCGucguGcGGCGUg -3' miRNA: 3'- aCGACGUC--------UGCGUUAGCGU----C-CCGUAg -5' |
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26037 | 3' | -55.7 | NC_005342.2 | + | 44982 | 0.66 | 0.587221 |
Target: 5'- cGCgGCGGACGCGccggcgagcGUCuacgGCAuuccguugacGGGCGUCa -3' miRNA: 3'- aCGaCGUCUGCGU---------UAG----CGU----------CCCGUAG- -5' |
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26037 | 3' | -55.7 | NC_005342.2 | + | 27010 | 0.66 | 0.576013 |
Target: 5'- uUGCguccgGCcccguGGCGUAGcCGCcGGGCGUCg -3' miRNA: 3'- -ACGa----CGu----CUGCGUUaGCGuCCCGUAG- -5' |
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26037 | 3' | -55.7 | NC_005342.2 | + | 13154 | 0.66 | 0.576013 |
Target: 5'- cGCcgacCGGGCGCGAUacgaucgugUGCAGGcGCGUCg -3' miRNA: 3'- aCGac--GUCUGCGUUA---------GCGUCC-CGUAG- -5' |
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26037 | 3' | -55.7 | NC_005342.2 | + | 17101 | 0.67 | 0.553752 |
Target: 5'- gGCgGC-GACGCGuUCGCcGGGC-UCg -3' miRNA: 3'- aCGaCGuCUGCGUuAGCGuCCCGuAG- -5' |
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26037 | 3' | -55.7 | NC_005342.2 | + | 36022 | 0.67 | 0.553752 |
Target: 5'- cGCcGCccgaGUAGUCGCcGGGCGUCg -3' miRNA: 3'- aCGaCGucugCGUUAGCGuCCCGUAG- -5' |
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26037 | 3' | -55.7 | NC_005342.2 | + | 36856 | 0.67 | 0.553752 |
Target: 5'- cGCcGCAcGACGCGAUCaCGuGcGGCAUCc -3' miRNA: 3'- aCGaCGU-CUGCGUUAGcGU-C-CCGUAG- -5' |
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26037 | 3' | -55.7 | NC_005342.2 | + | 4983 | 0.67 | 0.547122 |
Target: 5'- uUGCgccccgucucacugGCAGAUGCGG-CGC-GGGCAUg -3' miRNA: 3'- -ACGa-------------CGUCUGCGUUaGCGuCCCGUAg -5' |
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26037 | 3' | -55.7 | NC_005342.2 | + | 24623 | 0.67 | 0.536129 |
Target: 5'- cGC-GCAGGCgGCcaagaauucgaucacGGUCGCAGGGaCGUUg -3' miRNA: 3'- aCGaCGUCUG-CG---------------UUAGCGUCCC-GUAG- -5' |
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26037 | 3' | -55.7 | NC_005342.2 | + | 36415 | 0.67 | 0.531753 |
Target: 5'- aGC-GCAuGugGCAAUCGCGucugcgcaacgcGGGCuUCg -3' miRNA: 3'- aCGaCGU-CugCGUUAGCGU------------CCCGuAG- -5' |
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26037 | 3' | -55.7 | NC_005342.2 | + | 6582 | 0.67 | 0.531753 |
Target: 5'- cGCcGCccAGcgcCGCAGUCGCAGGuaAUCa -3' miRNA: 3'- aCGaCG--UCu--GCGUUAGCGUCCcgUAG- -5' |
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26037 | 3' | -55.7 | NC_005342.2 | + | 16922 | 0.67 | 0.531753 |
Target: 5'- gGCauUGCGGGCGCcg-UGCucGGCGUCg -3' miRNA: 3'- aCG--ACGUCUGCGuuaGCGucCCGUAG- -5' |
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26037 | 3' | -55.7 | NC_005342.2 | + | 1112 | 0.67 | 0.52848 |
Target: 5'- gUGCUGCGcgcGACGUugcacugcauauccGAUCGCGGcGGCuugcuUCg -3' miRNA: 3'- -ACGACGU---CUGCG--------------UUAGCGUC-CCGu----AG- -5' |
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26037 | 3' | -55.7 | NC_005342.2 | + | 14049 | 0.67 | 0.520871 |
Target: 5'- gGC-GCAGuAC-CAGUCGCaucguaccAGGGCAUCc -3' miRNA: 3'- aCGaCGUC-UGcGUUAGCG--------UCCCGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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