Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26043 | 3' | -53.7 | NC_005342.2 | + | 33456 | 0.66 | 0.794528 |
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Target: 5'- aGUUCGuGCGCGugaucaugucGUGcGAUGCGAcGUUc -3' miRNA: 3'- gCAAGC-CGCGC----------UAC-CUACGCUuCAGc -5' |
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| 26043 | 3' | -53.7 | NC_005342.2 | + | 25744 | 0.66 | 0.794528 |
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Target: 5'- --gUCGGCGUGAaGGucgguUGCGGgacguuGGUCGu -3' miRNA: 3'- gcaAGCCGCGCUaCCu----ACGCU------UCAGC- -5' |
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| 26043 | 3' | -53.7 | NC_005342.2 | + | 19185 | 0.66 | 0.764323 |
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Target: 5'- --gUCGGCGCGAUcgugGGcgGCGcAGUg- -3' miRNA: 3'- gcaAGCCGCGCUA----CCuaCGCuUCAgc -5' |
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| 26043 | 3' | -53.7 | NC_005342.2 | + | 37514 | 0.67 | 0.722167 |
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Target: 5'- gCGaagCGGCGCGAUGcGAaGCGc-GUCGc -3' miRNA: 3'- -GCaa-GCCGCGCUAC-CUaCGCuuCAGC- -5' |
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| 26043 | 3' | -53.7 | NC_005342.2 | + | 37745 | 0.67 | 0.721091 |
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Target: 5'- cCGUUCgauaccuGGCGCcccGAccUGGAUGCGAAG-Ca -3' miRNA: 3'- -GCAAG-------CCGCG---CU--ACCUACGCUUCaGc -5' |
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| 26043 | 3' | -53.7 | NC_005342.2 | + | 41388 | 0.69 | 0.600893 |
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Target: 5'- uGggCGGCGCGccGUGGcgGCGAug-CGg -3' miRNA: 3'- gCaaGCCGCGC--UACCuaCGCUucaGC- -5' |
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| 26043 | 3' | -53.7 | NC_005342.2 | + | 1844 | 0.7 | 0.524505 |
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Target: 5'- gGUUCuGGCGCGccuggcccGGAUugGCGggGUCGc -3' miRNA: 3'- gCAAG-CCGCGCua------CCUA--CGCuuCAGC- -5' |
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| 26043 | 3' | -53.7 | NC_005342.2 | + | 20970 | 0.7 | 0.524505 |
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Target: 5'- gCGcgCGGCGCGGgcgagUGGGUGCGuu-UCGa -3' miRNA: 3'- -GCaaGCCGCGCU-----ACCUACGCuucAGC- -5' |
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| 26043 | 3' | -53.7 | NC_005342.2 | + | 31992 | 0.72 | 0.43242 |
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Target: 5'- cCGgaUGGCGCGGgucGGcgGCGAuGUCGa -3' miRNA: 3'- -GCaaGCCGCGCUa--CCuaCGCUuCAGC- -5' |
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| 26043 | 3' | -53.7 | NC_005342.2 | + | 38757 | 1.1 | 0.001154 |
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Target: 5'- cCGUUCGGCGCGAUGGAUGCGAAGUCGg -3' miRNA: 3'- -GCAAGCCGCGCUACCUACGCUUCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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