Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26051 | 5' | -52.5 | NC_005342.2 | + | 40379 | 0.68 | 0.727302 |
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Target: 5'- uCGcAGGgcaucaucacGCAGGAGGACGUCcuGCGCa- -3' miRNA: 3'- -GCuUCCa---------CGUCCUUUUGCAG--CGCGac -5' |
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| 26051 | 5' | -52.5 | NC_005342.2 | + | 33660 | 0.68 | 0.716335 |
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Target: 5'- uCGAAGaaGCGGcGAAcGGCGcCGCGCUGa -3' miRNA: 3'- -GCUUCcaCGUC-CUU-UUGCaGCGCGAC- -5' |
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| 26051 | 5' | -52.5 | NC_005342.2 | + | 13171 | 0.68 | 0.694151 |
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Target: 5'- aCGAucguGUGCAGGc--GCGUCGCGUg- -3' miRNA: 3'- -GCUuc--CACGUCCuuuUGCAGCGCGac -5' |
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| 26051 | 5' | -52.5 | NC_005342.2 | + | 5284 | 0.69 | 0.649114 |
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Target: 5'- gCGAGGGUGCGGGuu-GCa--GCGCa- -3' miRNA: 3'- -GCUUCCACGUCCuuuUGcagCGCGac -5' |
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| 26051 | 5' | -52.5 | NC_005342.2 | + | 22968 | 0.7 | 0.59252 |
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Target: 5'- uGAcGGgcgGCGcGGAcgcAGACGUCGCGCUc -3' miRNA: 3'- gCUuCCa--CGU-CCU---UUUGCAGCGCGAc -5' |
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| 26051 | 5' | -52.5 | NC_005342.2 | + | 11017 | 0.71 | 0.558906 |
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Target: 5'- uCGuuGGUGCGGGccacAGCGUCGCGg-- -3' miRNA: 3'- -GCuuCCACGUCCuu--UUGCAGCGCgac -5' |
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| 26051 | 5' | -52.5 | NC_005342.2 | + | 40880 | 0.71 | 0.536793 |
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Target: 5'- gCGAAGGUGUAcGAucuGACGUCGgGCc- -3' miRNA: 3'- -GCUUCCACGUcCUu--UUGCAGCgCGac -5' |
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| 26051 | 5' | -52.5 | NC_005342.2 | + | 1820 | 0.74 | 0.384159 |
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Target: 5'- aGAGcGUGCAGGccGGCGUCGUGCg- -3' miRNA: 3'- gCUUcCACGUCCuuUUGCAGCGCGac -5' |
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| 26051 | 5' | -52.5 | NC_005342.2 | + | 36498 | 1.09 | 0.001524 |
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Target: 5'- gCGAAGGUGCAGGAAAACGUCGCGCUGa -3' miRNA: 3'- -GCUUCCACGUCCUUUUGCAGCGCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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