Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26056 | 3' | -54.8 | NC_005342.2 | + | 9603 | 0.66 | 0.691412 |
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Target: 5'- -aCCGGcugCCgGcGGCgcgCCUGCGCc -3' miRNA: 3'- caGGCCaaaGGgC-CCGaaaGGAUGCG- -5' |
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| 26056 | 3' | -54.8 | NC_005342.2 | + | 30526 | 0.67 | 0.658332 |
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Target: 5'- aUCCGGcg-CCCGGGCgacgUUUUgaucgucaGCGCg -3' miRNA: 3'- cAGGCCaaaGGGCCCGa---AAGGa-------UGCG- -5' |
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| 26056 | 3' | -54.8 | NC_005342.2 | + | 4272 | 0.67 | 0.647241 |
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Target: 5'- -cCCGGcucaUCCGGGUUcaCCUGCGUa -3' miRNA: 3'- caGGCCaaa-GGGCCCGAaaGGAUGCG- -5' |
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| 26056 | 3' | -54.8 | NC_005342.2 | + | 3161 | 0.68 | 0.613924 |
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Target: 5'- aGUCCGccGUUUucuugCCCGGGCgcggCC-GCGCg -3' miRNA: 3'- -CAGGC--CAAA-----GGGCCCGaaa-GGaUGCG- -5' |
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| 26056 | 3' | -54.8 | NC_005342.2 | + | 10381 | 0.68 | 0.580751 |
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Target: 5'- -aCCGGc--CUCGGGCUcgagUCCggACGCg -3' miRNA: 3'- caGGCCaaaGGGCCCGAa---AGGa-UGCG- -5' |
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| 26056 | 3' | -54.8 | NC_005342.2 | + | 25686 | 0.68 | 0.558838 |
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Target: 5'- -gUCGGcgUCUCGGGCUggCUUgGCGCa -3' miRNA: 3'- caGGCCaaAGGGCCCGAaaGGA-UGCG- -5' |
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| 26056 | 3' | -54.8 | NC_005342.2 | + | 27035 | 0.69 | 0.547967 |
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Target: 5'- -gCCGGg--CgUCGGGUUgcaaUCCUGCGCg -3' miRNA: 3'- caGGCCaaaG-GGCCCGAa---AGGAUGCG- -5' |
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| 26056 | 3' | -54.8 | NC_005342.2 | + | 16876 | 0.7 | 0.444032 |
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Target: 5'- aUCUGGUg-CCCGGGCg--CCcGCGUg -3' miRNA: 3'- cAGGCCAaaGGGCCCGaaaGGaUGCG- -5' |
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| 26056 | 3' | -54.8 | NC_005342.2 | + | 34297 | 1.12 | 0.000638 |
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Target: 5'- cGUCCGGUUUCCCGGGCUUUCCUACGCu -3' miRNA: 3'- -CAGGCCAAAGGGCCCGAAAGGAUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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