Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26089 | 3' | -55.7 | NC_005342.2 | + | 22061 | 0.66 | 0.632824 |
Target: 5'- gCAGGGCGGCgaagcgCGGCGc--UUGGCGc -3' miRNA: 3'- -GUCCCGCCGaa----GCCGCacaAGUUGUu -5' |
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26089 | 3' | -55.7 | NC_005342.2 | + | 3179 | 0.67 | 0.554547 |
Target: 5'- cCGGGcGCGGCcgCGcG-GUGUUCAGCGu -3' miRNA: 3'- -GUCC-CGCCGaaGC-CgCACAAGUUGUu -5' |
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26089 | 3' | -55.7 | NC_005342.2 | + | 22175 | 0.69 | 0.448668 |
Target: 5'- --cGGCGGCagcCGGCGgcgGUUCGGCGg -3' miRNA: 3'- gucCCGCCGaa-GCCGCa--CAAGUUGUu -5' |
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26089 | 3' | -55.7 | NC_005342.2 | + | 13337 | 0.7 | 0.381571 |
Target: 5'- aCGGGcGCGGCacgagCGGCGUGUcCAugAu -3' miRNA: 3'- -GUCC-CGCCGaa---GCCGCACAaGUugUu -5' |
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26089 | 3' | -55.7 | NC_005342.2 | + | 41427 | 0.7 | 0.372545 |
Target: 5'- -cGGGCGGCa-CGGCGcGaUCAACGAg -3' miRNA: 3'- guCCCGCCGaaGCCGCaCaAGUUGUU- -5' |
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26089 | 3' | -55.7 | NC_005342.2 | + | 16276 | 1.06 | 0.001053 |
Target: 5'- gCAGGGCGGCUUCGGCGUGUUCAACAAg -3' miRNA: 3'- -GUCCCGCCGAAGCCGCACAAGUUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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