Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2613 | 3' | -54.5 | NC_001488.1 | + | 1212 | 1.13 | 0.000048 |
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Target: 5'- uCCCUAUCUUACAUCCCCCAGGAGCCCc -3' miRNA: 3'- -GGGAUAGAAUGUAGGGGGUCCUCGGG- -5' |
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| 2613 | 3' | -54.5 | NC_001488.1 | + | 1286 | 0.68 | 0.174853 |
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Target: 5'- gCCUguaaGUCUUuCAUCUgCCAGG-GCCUa -3' miRNA: 3'- gGGA----UAGAAuGUAGGgGGUCCuCGGG- -5' |
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| 2613 | 3' | -54.5 | NC_001488.1 | + | 6833 | 0.66 | 0.236556 |
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Target: 5'- aCCCUgaagcaaGUCacaaaaccCAUCaaaaCCCAGGAGUCCu -3' miRNA: 3'- -GGGA-------UAGaau-----GUAGg---GGGUCCUCGGG- -5' |
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| 2613 | 3' | -54.5 | NC_001488.1 | + | 1711 | 0.66 | 0.25933 |
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Target: 5'- gCCC-AUCUUACGUUCCCUAGcGuacucaaacGCCa -3' miRNA: 3'- -GGGaUAGAAUGUAGGGGGUC-Cu--------CGGg -5' |
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| 2613 | 3' | -54.5 | NC_001488.1 | + | 2756 | 0.66 | 0.25933 |
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Target: 5'- aCCCUcaccUCU--CcUUCCCCAGG-GCCCc -3' miRNA: 3'- -GGGAu---AGAauGuAGGGGGUCCuCGGG- -5' |
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| 2613 | 3' | -54.5 | NC_001488.1 | + | 6387 | 0.66 | 0.274953 |
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Target: 5'- aUgUAUC---CGUCCUCCAGGAacgGCCCc -3' miRNA: 3'- gGgAUAGaauGUAGGGGGUCCU---CGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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