Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2617 | 5' | -53.1 | NC_001488.1 | + | 495 | 0.71 | 0.12511 |
Target: 5'- cUAAggGGCAGCCGagCUCGACCUgAg -3' miRNA: 3'- uGUUaaUCGUCGGCagGAGUUGGGgU- -5' |
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2617 | 5' | -53.1 | NC_001488.1 | + | 8676 | 0.71 | 0.12511 |
Target: 5'- cUAAggGGCAGCCGagCUCGACCUgAg -3' miRNA: 3'- uGUUaaUCGUCGGCagGAGUUGGGgU- -5' |
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2617 | 5' | -53.1 | NC_001488.1 | + | 7732 | 0.71 | 0.116719 |
Target: 5'- gACAAUUAGgAGCCuUCCUCAccaagguGCCUCu -3' miRNA: 3'- -UGUUAAUCgUCGGcAGGAGU-------UGGGGu -5' |
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2617 | 5' | -53.1 | NC_001488.1 | + | 3454 | 0.74 | 0.075712 |
Target: 5'- ------cGUGGCCG-CCUCAACCCCGc -3' miRNA: 3'- uguuaauCGUCGGCaGGAGUUGGGGU- -5' |
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2617 | 5' | -53.1 | NC_001488.1 | + | 2988 | 0.99 | 0.000696 |
Target: 5'- aACAAUU-GCAGCCGUCCUCAACCCCAu -3' miRNA: 3'- -UGUUAAuCGUCGGCAGGAGUUGGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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