Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26198 | 3' | -59 | NC_005342.2 | + | 6718 | 0.66 | 0.508978 |
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Target: 5'- -uUCGAGCGCCacGGCGccccggUCgcgcgCCUCGa -3' miRNA: 3'- gcAGCUCGCGGa-CCGCca----AGa----GGAGC- -5' |
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| 26198 | 3' | -59 | NC_005342.2 | + | 7289 | 0.66 | 0.507958 |
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Target: 5'- uCGUCGGGCGuugccgcgagaaaCCgccuucgGGCGGuUUCUUUUCGu -3' miRNA: 3'- -GCAGCUCGC-------------GGa------CCGCC-AAGAGGAGC- -5' |
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| 26198 | 3' | -59 | NC_005342.2 | + | 21819 | 0.66 | 0.49881 |
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Target: 5'- uGUCGAGC---UGGCGGUugagCUCgUCGa -3' miRNA: 3'- gCAGCUCGcggACCGCCAa---GAGgAGC- -5' |
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| 26198 | 3' | -59 | NC_005342.2 | + | 3591 | 0.66 | 0.49881 |
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Target: 5'- aGUCGGGCGCCUG-CGGg---CCg-- -3' miRNA: 3'- gCAGCUCGCGGACcGCCaagaGGagc -5' |
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| 26198 | 3' | -59 | NC_005342.2 | + | 30106 | 0.66 | 0.488733 |
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Target: 5'- gGcCGcGCGCCUGGCGGguggCgUCGc -3' miRNA: 3'- gCaGCuCGCGGACCGCCaagaGgAGC- -5' |
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| 26198 | 3' | -59 | NC_005342.2 | + | 30793 | 0.66 | 0.478752 |
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Target: 5'- aCG-CGAGCGCCU-GCaGGUUCggcgCCUgGc -3' miRNA: 3'- -GCaGCUCGCGGAcCG-CCAAGa---GGAgC- -5' |
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| 26198 | 3' | -59 | NC_005342.2 | + | 47788 | 0.66 | 0.468873 |
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Target: 5'- uGUUGGGCG-CUGGUGGUUUgugCUCa -3' miRNA: 3'- gCAGCUCGCgGACCGCCAAGag-GAGc -5' |
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| 26198 | 3' | -59 | NC_005342.2 | + | 15046 | 0.67 | 0.449435 |
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Target: 5'- gCGUCG-GCGUC-GGCGGUccgcuaaccggCUUCUCGa -3' miRNA: 3'- -GCAGCuCGCGGaCCGCCAa----------GAGGAGC- -5' |
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| 26198 | 3' | -59 | NC_005342.2 | + | 46000 | 0.72 | 0.225544 |
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Target: 5'- -uUCGAGCGCCUGGCGcugaUCCggcgCGa -3' miRNA: 3'- gcAGCUCGCGGACCGCcaagAGGa---GC- -5' |
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| 26198 | 3' | -59 | NC_005342.2 | + | 30567 | 1.09 | 0.000399 |
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Target: 5'- gCGUCGAGCGCCUGGCGGUUCUCCUCGg -3' miRNA: 3'- -GCAGCUCGCGGACCGCCAAGAGGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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