Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2621 | 5' | -51.5 | NC_001491.2 | + | 7205 | 0.66 | 0.982505 |
Target: 5'- cUCCCAauauucaaaugacauGaggGGGCGUGGCUUGAg----- -3' miRNA: 3'- -AGGGU---------------Ca--CCCGCACCGGAUUauuuuu -5' |
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2621 | 5' | -51.5 | NC_001491.2 | + | 147392 | 0.66 | 0.979418 |
Target: 5'- aCCCAGUGGGCaauaaGcGCCUcgggcGUGAAGAc -3' miRNA: 3'- aGGGUCACCCGca---C-CGGAu----UAUUUUU- -5' |
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2621 | 5' | -51.5 | NC_001491.2 | + | 18 | 0.66 | 0.976977 |
Target: 5'- aCCCccgcauuugaauAGggGGGCGUGGUCUAAgggGGGGGg -3' miRNA: 3'- aGGG------------UCa-CCCGCACCGGAUUa--UUUUU- -5' |
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2621 | 5' | -51.5 | NC_001491.2 | + | 88525 | 0.66 | 0.97433 |
Target: 5'- uUCCCAGcgggugGGGgGUGGCg-AGUAAGu- -3' miRNA: 3'- -AGGGUCa-----CCCgCACCGgaUUAUUUuu -5' |
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2621 | 5' | -51.5 | NC_001491.2 | + | 23790 | 0.7 | 0.898976 |
Target: 5'- aUUCCGaugccGUGGGCGUGuGCCU-AUAAAGc -3' miRNA: 3'- -AGGGU-----CACCCGCAC-CGGAuUAUUUUu -5' |
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2621 | 5' | -51.5 | NC_001491.2 | + | 43086 | 0.7 | 0.884905 |
Target: 5'- gCUUAGUGGGUggggGUGGCC-AGUAGAGGu -3' miRNA: 3'- aGGGUCACCCG----CACCGGaUUAUUUUU- -5' |
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2621 | 5' | -51.5 | NC_001491.2 | + | 6330 | 0.71 | 0.861954 |
Target: 5'- cCCUAGUGGGaGUGGCCa-------- -3' miRNA: 3'- aGGGUCACCCgCACCGGauuauuuuu -5' |
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2621 | 5' | -51.5 | NC_001491.2 | + | 85 | 0.71 | 0.853836 |
Target: 5'- gUCaCCGGaagGGGCGUGGCCg---GAAGc -3' miRNA: 3'- -AG-GGUCa--CCCGCACCGGauuaUUUUu -5' |
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2621 | 5' | -51.5 | NC_001491.2 | + | 149918 | 0.93 | 0.069825 |
Target: 5'- cUCCCAGUGGGCGUGGCCUcccAGUGGGc- -3' miRNA: 3'- -AGGGUCACCCGCACCGGA---UUAUUUuu -5' |
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2621 | 5' | -51.5 | NC_001491.2 | + | 149884 | 0.93 | 0.069825 |
Target: 5'- cUCCCAGUGGGCGUGGCCUcccAGUGGGc- -3' miRNA: 3'- -AGGGUCACCCGCACCGGA---UUAUUUuu -5' |
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2621 | 5' | -51.5 | NC_001491.2 | + | 149850 | 0.93 | 0.069825 |
Target: 5'- cUCCCAGUGGGCGUGGCCUcccAGUGGGc- -3' miRNA: 3'- -AGGGUCACCCGCACCGGA---UUAUUUuu -5' |
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2621 | 5' | -51.5 | NC_001491.2 | + | 149816 | 0.93 | 0.069825 |
Target: 5'- cUCCCAGUGGGCGUGGCCUcccAGUGGGc- -3' miRNA: 3'- -AGGGUCACCCGCACCGGA---UUAUUUuu -5' |
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2621 | 5' | -51.5 | NC_001491.2 | + | 149782 | 0.93 | 0.069825 |
Target: 5'- cUCCCAGUGGGCGUGGCCUcccAGUGGGc- -3' miRNA: 3'- -AGGGUCACCCGCACCGGA---UUAUUUuu -5' |
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2621 | 5' | -51.5 | NC_001491.2 | + | 149748 | 0.93 | 0.069825 |
Target: 5'- cUCCCAGUGGGCGUGGCCUcccAGUGGGc- -3' miRNA: 3'- -AGGGUCACCCGCACCGGA---UUAUUUuu -5' |
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2621 | 5' | -51.5 | NC_001491.2 | + | 149952 | 1.07 | 0.008018 |
Target: 5'- cUCCCAGUGGGCGUGGCCUAAUAAAAAa -3' miRNA: 3'- -AGGGUCACCCGCACCGGAUUAUUUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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