Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26221 | 5' | -56 | NC_005342.2 | + | 13226 | 0.69 | 0.441527 |
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Target: 5'- uGGUGuUCGGCGCGAccGGCAAUGgUaCGGc -3' miRNA: 3'- cUCAU-AGCCGCGCU--CCGUUGCgA-GCU- -5' |
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| 26221 | 5' | -56 | NC_005342.2 | + | 19184 | 0.7 | 0.385076 |
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Target: 5'- ---gGUCGGCGCGAucgugGGCGgcgcagugcucGCGCUCGu -3' miRNA: 3'- cucaUAGCCGCGCU-----CCGU-----------UGCGAGCu -5' |
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| 26221 | 5' | -56 | NC_005342.2 | + | 19489 | 0.71 | 0.350191 |
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Target: 5'- ---gAUCGGCGCuggcgcgcuGGCGGCGCUCGu -3' miRNA: 3'- cucaUAGCCGCGcu-------CCGUUGCGAGCu -5' |
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| 26221 | 5' | -56 | NC_005342.2 | + | 5527 | 0.71 | 0.341828 |
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Target: 5'- -cGgcUUGGCGCGAGuGCAGaccguucaGCUCGAu -3' miRNA: 3'- cuCauAGCCGCGCUC-CGUUg-------CGAGCU- -5' |
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| 26221 | 5' | -56 | NC_005342.2 | + | 34226 | 0.71 | 0.325539 |
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Target: 5'- cGAG---CGGCGCGcGGCGuccuacGCGCUCGAc -3' miRNA: 3'- -CUCauaGCCGCGCuCCGU------UGCGAGCU- -5' |
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| 26221 | 5' | -56 | NC_005342.2 | + | 41448 | 0.72 | 0.309833 |
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Target: 5'- cGAGgagAUCGGCGCG-GGCGacucgcGCGC-CGGg -3' miRNA: 3'- -CUCa--UAGCCGCGCuCCGU------UGCGaGCU- -5' |
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| 26221 | 5' | -56 | NC_005342.2 | + | 17811 | 0.72 | 0.287369 |
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Target: 5'- -----gUGGCGCGAGGCAGuCGCUaCGGc -3' miRNA: 3'- cucauaGCCGCGCUCCGUU-GCGA-GCU- -5' |
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| 26221 | 5' | -56 | NC_005342.2 | + | 20715 | 0.74 | 0.239974 |
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Target: 5'- cGAGgcGUCGGCGC-AGGCAgACGCUgCGAu -3' miRNA: 3'- -CUCa-UAGCCGCGcUCCGU-UGCGA-GCU- -5' |
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| 26221 | 5' | -56 | NC_005342.2 | + | 42816 | 1.09 | 0.00075 |
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Target: 5'- cGAGUAUCGGCGCGAGGCAACGCUCGAc -3' miRNA: 3'- -CUCAUAGCCGCGCUCCGUUGCGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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