Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26225 | 3' | -58.5 | NC_005342.2 | + | 24291 | 0.66 | 0.478941 |
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Target: 5'- -cGGCGACUGCgCGACCU---GGAAc- -3' miRNA: 3'- uaCCGCUGGCG-GCUGGAcacCCUUau -5' |
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| 26225 | 3' | -58.5 | NC_005342.2 | + | 44300 | 0.68 | 0.339986 |
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Target: 5'- --cGCGAUgGUCGGCgUGUGGGAGa- -3' miRNA: 3'- uacCGCUGgCGGCUGgACACCCUUau -5' |
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| 26225 | 3' | -58.5 | NC_005342.2 | + | 45590 | 0.69 | 0.323806 |
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Target: 5'- -aGGCGACCGCCGgcGCCgucaUGGGc--- -3' miRNA: 3'- uaCCGCUGGCGGC--UGGac--ACCCuuau -5' |
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| 26225 | 3' | -58.5 | NC_005342.2 | + | 33609 | 0.7 | 0.245132 |
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Target: 5'- -aGGCGAUCGCCGACCUGa------- -3' miRNA: 3'- uaCCGCUGGCGGCUGGACacccuuau -5' |
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| 26225 | 3' | -58.5 | NC_005342.2 | + | 9535 | 0.72 | 0.203798 |
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Target: 5'- -aGGCGAgCGCCGGCggCUGcGGGAAUu -3' miRNA: 3'- uaCCGCUgGCGGCUG--GACaCCCUUAu -5' |
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| 26225 | 3' | -58.5 | NC_005342.2 | + | 43919 | 1.04 | 0.000758 |
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Target: 5'- cAUGGCGACCGCCGACCUGUGGGAAUAc -3' miRNA: 3'- -UACCGCUGGCGGCUGGACACCCUUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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