Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26227 | 3' | -53.9 | NC_005342.2 | + | 20332 | 0.66 | 0.774693 |
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Target: 5'- ---cGGCCG-UGCCGAcGGguacCGCGAc- -3' miRNA: 3'- agaaCCGGCaAUGGCU-CCa---GCGCUuc -5' |
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| 26227 | 3' | -53.9 | NC_005342.2 | + | 43468 | 0.67 | 0.711249 |
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Target: 5'- ---gGGCuaCGaUGCCGAGGUCGCa--- -3' miRNA: 3'- agaaCCG--GCaAUGGCUCCAGCGcuuc -5' |
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| 26227 | 3' | -53.9 | NC_005342.2 | + | 2560 | 0.67 | 0.700325 |
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Target: 5'- ---cGGCCGU--CCaGGcGGUCGUGAAGg -3' miRNA: 3'- agaaCCGGCAauGG-CU-CCAGCGCUUC- -5' |
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| 26227 | 3' | -53.9 | NC_005342.2 | + | 2677 | 0.67 | 0.700325 |
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Target: 5'- ---cGGCCGacagGCCGAGcucGUCgGCGAGGu -3' miRNA: 3'- agaaCCGGCaa--UGGCUC---CAG-CGCUUC- -5' |
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| 26227 | 3' | -53.9 | NC_005342.2 | + | 22654 | 0.68 | 0.644913 |
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Target: 5'- ---cGGCCGUcGCCGGcgucggcccGGUUGCGAc- -3' miRNA: 3'- agaaCCGGCAaUGGCU---------CCAGCGCUuc -5' |
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| 26227 | 3' | -53.9 | NC_005342.2 | + | 9338 | 0.71 | 0.491851 |
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Target: 5'- -gUUGGCCG-UGCCGAucagCGCGAAGc -3' miRNA: 3'- agAACCGGCaAUGGCUcca-GCGCUUC- -5' |
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| 26227 | 3' | -53.9 | NC_005342.2 | + | 44165 | 0.72 | 0.409173 |
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Target: 5'- gCUUGGCCugccggugcagaaaGaUACCGAGGgUGCGAAGu -3' miRNA: 3'- aGAACCGG--------------CaAUGGCUCCaGCGCUUC- -5' |
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| 26227 | 3' | -53.9 | NC_005342.2 | + | 27905 | 0.74 | 0.316355 |
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Target: 5'- ---cGGCCGcaAUCGAGGUUGCGAGa -3' miRNA: 3'- agaaCCGGCaaUGGCUCCAGCGCUUc -5' |
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| 26227 | 3' | -53.9 | NC_005342.2 | + | 8519 | 0.74 | 0.316355 |
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Target: 5'- gUCUUuGCCGagcUUGCCGAGGUCGCu--- -3' miRNA: 3'- -AGAAcCGGC---AAUGGCUCCAGCGcuuc -5' |
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| 26227 | 3' | -53.9 | NC_005342.2 | + | 44490 | 1.1 | 0.001054 |
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Target: 5'- aUCUUGGCCGUUACCGAGGUCGCGAAGa -3' miRNA: 3'- -AGAACCGGCAAUGGCUCCAGCGCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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