Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26233 | 3' | -62.2 | NC_005342.2 | + | 35601 | 0.7 | 0.147596 |
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Target: 5'- cGCCg-GGCgGGGCGCGG-GCGgGCg -3' miRNA: 3'- uUGGaaCCGgCUCGCGCCaCGCgCGa -5' |
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| 26233 | 3' | -62.2 | NC_005342.2 | + | 34102 | 0.72 | 0.11541 |
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Target: 5'- uGCC--GGCCGAcuucGCGCGGcgcgGCGCGCUc -3' miRNA: 3'- uUGGaaCCGGCU----CGCGCCa---CGCGCGA- -5' |
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| 26233 | 3' | -62.2 | NC_005342.2 | + | 2410 | 0.72 | 0.103315 |
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Target: 5'- uGCCUUGGUacGGCGCGGcgaacacuUGCGCGCc -3' miRNA: 3'- uUGGAACCGgcUCGCGCC--------ACGCGCGa -5' |
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| 26233 | 3' | -62.2 | NC_005342.2 | + | 13934 | 0.75 | 0.067814 |
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Target: 5'- cGACUUcgGGCCG-GCGgcCGGUGCGCGCg -3' miRNA: 3'- -UUGGAa-CCGGCuCGC--GCCACGCGCGa -5' |
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| 26233 | 3' | -62.2 | NC_005342.2 | + | 91 | 0.77 | 0.042962 |
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Target: 5'- cACCau-GCCG-GCGCGGUGCGCGCg -3' miRNA: 3'- uUGGaacCGGCuCGCGCCACGCGCGa -5' |
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| 26233 | 3' | -62.2 | NC_005342.2 | + | 45820 | 1.05 | 0.000282 |
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Target: 5'- cAACCUUGGCCGAGCGCGGUGCGCGCUa -3' miRNA: 3'- -UUGGAACCGGCUCGCGCCACGCGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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