Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26242 | 5' | -53.6 | NC_005344.1 | + | 11825 | 0.66 | 0.713388 |
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Target: 5'- ---cAGGCUGGCGuGAacguggcaaacauaCCUGCAUCa- -3' miRNA: 3'- guuuUCCGACCGUuCU--------------GGACGUGGcc -5' |
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| 26242 | 5' | -53.6 | NC_005344.1 | + | 11078 | 0.66 | 0.705539 |
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Target: 5'- gCAAAAGGCUGaCAuaGCCcGCGCUGa -3' miRNA: 3'- -GUUUUCCGACcGUucUGGaCGUGGCc -5' |
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| 26242 | 5' | -53.6 | NC_005344.1 | + | 15283 | 0.66 | 0.694259 |
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Target: 5'- ----uGGCUGGCAuuACCgaggUGCagaaACCGGa -3' miRNA: 3'- guuuuCCGACCGUucUGG----ACG----UGGCC- -5' |
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| 26242 | 5' | -53.6 | NC_005344.1 | + | 14368 | 0.67 | 0.625643 |
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Target: 5'- aGAGGGGUUGGUAAGGCaucGCAacuCCGa -3' miRNA: 3'- gUUUUCCGACCGUUCUGga-CGU---GGCc -5' |
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| 26242 | 5' | -53.6 | NC_005344.1 | + | 10688 | 0.68 | 0.59126 |
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Target: 5'- --cGAGGCc-GCAAGGCgUGCGCCa- -3' miRNA: 3'- guuUUCCGacCGUUCUGgACGUGGcc -5' |
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| 26242 | 5' | -53.6 | NC_005344.1 | + | 10192 | 0.68 | 0.579861 |
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Target: 5'- -uGGGGaGCUGGCAuuGGugCUGgUAUCGGa -3' miRNA: 3'- guUUUC-CGACCGU--UCugGAC-GUGGCC- -5' |
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| 26242 | 5' | -53.6 | NC_005344.1 | + | 10215 | 1.1 | 0.00077 |
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Target: 5'- cCAAAAGGCUGGCAAGACCUGCACCGGc -3' miRNA: 3'- -GUUUUCCGACCGUUCUGGACGUGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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