Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26243 | 5' | -54.5 | NC_005344.1 | + | 32979 | 0.67 | 0.544675 |
Target: 5'- -uGGCGUGAGCu--GCCUCGCUGaaGCUu -3' miRNA: 3'- agUUGCGCUCGuacUGGAGUGAC--CGA- -5' |
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26243 | 5' | -54.5 | NC_005344.1 | + | 7643 | 0.68 | 0.482571 |
Target: 5'- cCAGCGCGAGCAuUGAgaaaauccuccgcuCCUacacugcugaugaACUGGCUg -3' miRNA: 3'- aGUUGCGCUCGU-ACU--------------GGAg------------UGACCGA- -5' |
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26243 | 5' | -54.5 | NC_005344.1 | + | 31981 | 0.81 | 0.074988 |
Target: 5'- uUCGGCaGCGAGCgacaaaaaugcucuGUGugCUCACUGGCUa -3' miRNA: 3'- -AGUUG-CGCUCG--------------UACugGAGUGACCGA- -5' |
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26243 | 5' | -54.5 | NC_005344.1 | + | 16743 | 1.09 | 0.000717 |
Target: 5'- cUCAACGCGAGCAUGACCUCACUGGCUg -3' miRNA: 3'- -AGUUGCGCUCGUACUGGAGUGACCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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