miRNA: 3'- -CUGcCCAUUuuUCACCUAu------UUUAUUA- -5'
miRNA: 3'- cUGCCCA-----------UUUUUCACCUAUuu-UAUUa -5'
miRNA: 3'- cUGCCCAUUUU-UCaCCUAUUUUAUua -5'
miRNA: 3'- cUGCCCAUUUU----UCACCUAUUUUAuua -5'
miRNA: 3'- -CUGCCCAUUUUUCACCUAUUUUAUUA- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26244 | 3' | -39.5 | NC_005344.1 | + | 28898 | 0.66 | 0.999999 |
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Target: 5'- aGGC-GGUGAuuGGUGGAUugcuucuGGAUGAUg -3' miRNA: 3'- -CUGcCCAUUuuUCACCUAu------UUUAUUA- -5' |
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| 26244 | 3' | -39.5 | NC_005344.1 | + | 20664 | 0.7 | 0.999517 |
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Target: 5'- cGCGGGUcgauauauucaGGGAAGUGGGUAuauGUGGc -3' miRNA: 3'- cUGCCCA-----------UUUUUCACCUAUuu-UAUUa -5' |
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| 26244 | 3' | -39.5 | NC_005344.1 | + | 18216 | 0.71 | 0.998692 |
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Target: 5'- aAUGGGUAAAAuAGcGGGUAAAAUAu- -3' miRNA: 3'- cUGCCCAUUUU-UCaCCUAUUUUAUua -5' |
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| 26244 | 3' | -39.5 | NC_005344.1 | + | 17919 | 0.76 | 0.953832 |
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Target: 5'- cAUGGGUAAAAauccGGUGGGUAAAAUu-- -3' miRNA: 3'- cUGCCCAUUUU----UCACCUAUUUUAuua -5' |
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| 26244 | 3' | -39.5 | NC_005344.1 | + | 18008 | 1.07 | 0.045179 |
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Target: 5'- uGACGGGUAAAAAGUGGAUAAAAUAAUu -3' miRNA: 3'- -CUGCCCAUUUUUCACCUAUUUUAUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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