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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26247 | 5' | -51.3 | NC_005344.1 | + | 37453 | 0.66 | 0.791044 |
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Target: 5'- -cGCGUuagCAUCAGCAagcuccuuuguguAUCUGGCGuCa -3' miRNA: 3'- uaCGCAa--GUAGUCGU-------------UAGGCCGUuGc -5' |
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| 26247 | 5' | -51.3 | NC_005344.1 | + | 7591 | 0.68 | 0.715185 |
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Target: 5'- -gGCGUUUugCAGUAAgucCCGGCGAUGc -3' miRNA: 3'- uaCGCAAGuaGUCGUUa--GGCCGUUGC- -5' |
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| 26247 | 5' | -51.3 | NC_005344.1 | + | 1280 | 0.68 | 0.715185 |
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Target: 5'- uUGaUGUcCAUCAGCAGaccUUCGGCAAUGc -3' miRNA: 3'- uAC-GCAaGUAGUCGUU---AGGCCGUUGC- -5' |
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| 26247 | 5' | -51.3 | NC_005344.1 | + | 34428 | 0.71 | 0.483583 |
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Target: 5'- uUGCGUUCAUCaaaucggaguugaGGCGccgcagcaguuGUCCGGUAAUGc -3' miRNA: 3'- uACGCAAGUAG-------------UCGU-----------UAGGCCGUUGC- -5' |
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| 26247 | 5' | -51.3 | NC_005344.1 | + | 7567 | 1.08 | 0.001633 |
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Target: 5'- uAUGCGUUCAUCAGCAAUCCGGCAACGg -3' miRNA: 3'- -UACGCAAGUAGUCGUUAGGCCGUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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