Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26248 | 3' | -49.9 | NC_005344.1 | + | 12306 | 0.66 | 0.870261 |
Target: 5'- cGCCACGAuaaGCAGCACcuaauCCAc -3' miRNA: 3'- aCGGUGUUuacCGUCGUGcaauuGGU- -5' |
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26248 | 3' | -49.9 | NC_005344.1 | + | 1372 | 0.67 | 0.813934 |
Target: 5'- cGCCACGAuguucaagGGCAGCGaauc-GCCAu -3' miRNA: 3'- aCGGUGUUua------CCGUCGUgcaauUGGU- -5' |
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26248 | 3' | -49.9 | NC_005344.1 | + | 36523 | 0.67 | 0.793242 |
Target: 5'- cGCgGCAgauauAAUGGCGGUGCGUUuACa- -3' miRNA: 3'- aCGgUGU-----UUACCGUCGUGCAAuUGgu -5' |
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26248 | 3' | -49.9 | NC_005344.1 | + | 37298 | 0.68 | 0.760764 |
Target: 5'- cGCCuACAAAgaGCAGCGCGaUAaaGCCAc -3' miRNA: 3'- aCGG-UGUUUacCGUCGUGCaAU--UGGU- -5' |
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26248 | 3' | -49.9 | NC_005344.1 | + | 36137 | 0.69 | 0.692036 |
Target: 5'- cGCCuuguaaGCGAAUgGGUAGCGguucaagucCGUUAACCAg -3' miRNA: 3'- aCGG------UGUUUA-CCGUCGU---------GCAAUUGGU- -5' |
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26248 | 3' | -49.9 | NC_005344.1 | + | 9732 | 0.71 | 0.597077 |
Target: 5'- cGCCucuuGCGGA--GCAGUAUGUUAACCAg -3' miRNA: 3'- aCGG----UGUUUacCGUCGUGCAAUUGGU- -5' |
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26248 | 3' | -49.9 | NC_005344.1 | + | 37523 | 0.72 | 0.526932 |
Target: 5'- uUG-CACAGGUGGCGGCgacuugcagGCGUUuacGACCAg -3' miRNA: 3'- -ACgGUGUUUACCGUCG---------UGCAA---UUGGU- -5' |
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26248 | 3' | -49.9 | NC_005344.1 | + | 3651 | 0.79 | 0.214173 |
Target: 5'- aGgCGCAAAUGGCAGCACaa-AGCCAg -3' miRNA: 3'- aCgGUGUUUACCGUCGUGcaaUUGGU- -5' |
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26248 | 3' | -49.9 | NC_005344.1 | + | 9008 | 1.1 | 0.001755 |
Target: 5'- uUGCCACAAAUGGCAGCACGUUAACCAu -3' miRNA: 3'- -ACGGUGUUUACCGUCGUGCAAUUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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