Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26249 | 5' | -47.6 | NC_005344.1 | + | 1190 | 0.66 | 0.956573 |
Target: 5'- -gGGUCAUG--UGCAGAaacaACAGCGCCu -3' miRNA: 3'- cgUUAGUACcgACGUUU----UGUUGCGGu -5' |
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26249 | 5' | -47.6 | NC_005344.1 | + | 33220 | 0.66 | 0.953832 |
Target: 5'- cGCuuUCAUGGUgagccugcuguccgGCGAgaacGACAugGCCc -3' miRNA: 3'- -CGuuAGUACCGa-------------CGUU----UUGUugCGGu -5' |
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26249 | 5' | -47.6 | NC_005344.1 | + | 10245 | 0.66 | 0.941711 |
Target: 5'- gGUAAUCAUGGCUacuugGCAAGGaucaaAugGCgGa -3' miRNA: 3'- -CGUUAGUACCGA-----CGUUUUg----UugCGgU- -5' |
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26249 | 5' | -47.6 | NC_005344.1 | + | 13959 | 0.67 | 0.936103 |
Target: 5'- aGCAAggccauUCAUGGCUGUAu-AUGAUGCg- -3' miRNA: 3'- -CGUU------AGUACCGACGUuuUGUUGCGgu -5' |
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26249 | 5' | -47.6 | NC_005344.1 | + | 37354 | 0.67 | 0.936103 |
Target: 5'- gGCAA-CAUGauGUaGCAGAACuuGACGCCAg -3' miRNA: 3'- -CGUUaGUAC--CGaCGUUUUG--UUGCGGU- -5' |
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26249 | 5' | -47.6 | NC_005344.1 | + | 35055 | 0.67 | 0.930164 |
Target: 5'- uCAAUCAcUGGCUGCuuAAUGGCGgUg -3' miRNA: 3'- cGUUAGU-ACCGACGuuUUGUUGCgGu -5' |
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26249 | 5' | -47.6 | NC_005344.1 | + | 13261 | 0.67 | 0.930164 |
Target: 5'- cGCAG-CA-GGCUGCAAaagcggcaguAACAACaCCAa -3' miRNA: 3'- -CGUUaGUaCCGACGUU----------UUGUUGcGGU- -5' |
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26249 | 5' | -47.6 | NC_005344.1 | + | 9908 | 0.68 | 0.903084 |
Target: 5'- aGCAAUCAacUGGCUaCGAuc--GCGCCGa -3' miRNA: 3'- -CGUUAGU--ACCGAcGUUuuguUGCGGU- -5' |
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26249 | 5' | -47.6 | NC_005344.1 | + | 37763 | 0.68 | 0.895493 |
Target: 5'- cGCAGUaaggGGCUGCGuugAGAUAAgaGCCAg -3' miRNA: 3'- -CGUUAgua-CCGACGU---UUUGUUg-CGGU- -5' |
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26249 | 5' | -47.6 | NC_005344.1 | + | 31869 | 0.68 | 0.875978 |
Target: 5'- -aGAUCG-GGCUGCAuGACuaucaaaucaaauACGCCAg -3' miRNA: 3'- cgUUAGUaCCGACGUuUUGu------------UGCGGU- -5' |
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26249 | 5' | -47.6 | NC_005344.1 | + | 7551 | 0.69 | 0.856552 |
Target: 5'- uCAGUgAUGGCUGUgcgacauacAAAGCAgcaccagcggugguuGCGCCAg -3' miRNA: 3'- cGUUAgUACCGACG---------UUUUGU---------------UGCGGU- -5' |
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26249 | 5' | -47.6 | NC_005344.1 | + | 3119 | 0.7 | 0.781897 |
Target: 5'- gGCAGUCA-GGCcaUGCAGca-GAUGCCAa -3' miRNA: 3'- -CGUUAGUaCCG--ACGUUuugUUGCGGU- -5' |
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26249 | 5' | -47.6 | NC_005344.1 | + | 10664 | 1.12 | 0.002134 |
Target: 5'- uGCAAUCAUGGCUGCAAAACAACGCCAa -3' miRNA: 3'- -CGUUAGUACCGACGUUUUGUUGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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