Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26252 | 5' | -51.1 | NC_005344.1 | + | 21739 | 0.66 | 0.848251 |
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Target: 5'- aCUGGCagAAAUUGcCAGCGAAuaucugcgaaaaGGCUc -3' miRNA: 3'- -GACCGagUUUAGCaGUCGCUU------------UCGGc -5' |
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| 26252 | 5' | -51.1 | NC_005344.1 | + | 4258 | 0.66 | 0.848251 |
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Target: 5'- gUGGUgcagguugcccgUCAAugugaucaucAUCGUCAGCGuuAAGCUGa -3' miRNA: 3'- gACCG------------AGUU----------UAGCAGUCGCu-UUCGGC- -5' |
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| 26252 | 5' | -51.1 | NC_005344.1 | + | 4641 | 0.67 | 0.7834 |
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Target: 5'- aUGGCUUAGGUaagagccagcaauuaCGcCAGCGuguAGCCGc -3' miRNA: 3'- gACCGAGUUUA---------------GCaGUCGCuu-UCGGC- -5' |
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| 26252 | 5' | -51.1 | NC_005344.1 | + | 20251 | 0.67 | 0.768534 |
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Target: 5'- uUGGCUUuAcgCGUUGGCGAAuaaucauuucuGCCGg -3' miRNA: 3'- gACCGAGuUuaGCAGUCGCUUu----------CGGC- -5' |
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| 26252 | 5' | -51.1 | NC_005344.1 | + | 6469 | 0.7 | 0.632291 |
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Target: 5'- -cGGCUCAAcAUCaGUCAGCGAugcauuagAAGCa- -3' miRNA: 3'- gaCCGAGUU-UAG-CAGUCGCU--------UUCGgc -5' |
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| 26252 | 5' | -51.1 | NC_005344.1 | + | 29795 | 1.09 | 0.001713 |
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Target: 5'- uCUGGCUCAAAUCGUCAGCGAAAGCCGu -3' miRNA: 3'- -GACCGAGUUUAGCAGUCGCUUUCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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