Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26256 | 5' | -49.1 | NC_005345.2 | + | 36365 | 0.66 | 0.957961 |
Target: 5'- -gGCGGGUCguuGCGG--GUGG--UCCa -3' miRNA: 3'- agCGCCCAGau-UGCUuuUACCugAGG- -5' |
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26256 | 5' | -49.1 | NC_005345.2 | + | 44826 | 0.66 | 0.953667 |
Target: 5'- cUCGCGGGUggGGCGcaucGAcgGGugcuugauCUCCg -3' miRNA: 3'- -AGCGCCCAgaUUGCu---UUuaCCu-------GAGG- -5' |
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26256 | 5' | -49.1 | NC_005345.2 | + | 23204 | 0.67 | 0.949087 |
Target: 5'- cUGCGGGaCUggGACGAGAucguGUGGGCg-- -3' miRNA: 3'- aGCGCCCaGA--UUGCUUU----UACCUGagg -5' |
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26256 | 5' | -49.1 | NC_005345.2 | + | 4237 | 0.67 | 0.949087 |
Target: 5'- cCGauGGUCgccgcuGCGGAGcgGGACgaggCCa -3' miRNA: 3'- aGCgcCCAGau----UGCUUUuaCCUGa---GG- -5' |
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26256 | 5' | -49.1 | NC_005345.2 | + | 31548 | 0.67 | 0.949087 |
Target: 5'- cUGgGGGUCgAGCGAGGA-GGACg-- -3' miRNA: 3'- aGCgCCCAGaUUGCUUUUaCCUGagg -5' |
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26256 | 5' | -49.1 | NC_005345.2 | + | 26774 | 0.67 | 0.944216 |
Target: 5'- gCGCGGGUgcGGCGGAucGUGGcCggcgCCg -3' miRNA: 3'- aGCGCCCAgaUUGCUUu-UACCuGa---GG- -5' |
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26256 | 5' | -49.1 | NC_005345.2 | + | 16069 | 0.67 | 0.933587 |
Target: 5'- aUCGCGGGgu---CGGuguGAUGGACaCCc -3' miRNA: 3'- -AGCGCCCagauuGCUu--UUACCUGaGG- -5' |
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26256 | 5' | -49.1 | NC_005345.2 | + | 5282 | 0.67 | 0.927824 |
Target: 5'- cUCGgGGGUCggguacuGCGggGGgcGGGC-CCa -3' miRNA: 3'- -AGCgCCCAGau-----UGCuuUUa-CCUGaGG- -5' |
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26256 | 5' | -49.1 | NC_005345.2 | + | 15145 | 0.68 | 0.915399 |
Target: 5'- cUGCGGGUCgGGCGGGuucacgaccacGAUGGgguuGCUCa -3' miRNA: 3'- aGCGCCCAGaUUGCUU-----------UUACC----UGAGg -5' |
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26256 | 5' | -49.1 | NC_005345.2 | + | 39595 | 0.68 | 0.90107 |
Target: 5'- gCGCGGGgacuACGAGGggcagcucggcguGUGGAC-CCg -3' miRNA: 3'- aGCGCCCagauUGCUUU-------------UACCUGaGG- -5' |
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26256 | 5' | -49.1 | NC_005345.2 | + | 16591 | 0.68 | 0.894533 |
Target: 5'- gCGCGGGUC-AACGGAcuggugagaGAcGGGCggcCCg -3' miRNA: 3'- aGCGCCCAGaUUGCUU---------UUaCCUGa--GG- -5' |
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26256 | 5' | -49.1 | NC_005345.2 | + | 17291 | 0.69 | 0.886997 |
Target: 5'- gCGCGGGUCUuguCGGccGGAcGGGCgaacCCg -3' miRNA: 3'- aGCGCCCAGAuu-GCU--UUUaCCUGa---GG- -5' |
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26256 | 5' | -49.1 | NC_005345.2 | + | 979 | 0.69 | 0.886997 |
Target: 5'- cCGCGGGUC-AAUGAGcuc-GugUCCg -3' miRNA: 3'- aGCGCCCAGaUUGCUUuuacCugAGG- -5' |
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26256 | 5' | -49.1 | NC_005345.2 | + | 2060 | 0.69 | 0.871083 |
Target: 5'- -gGCGGGcCgcgGCGAAAAUGcGCUCUu -3' miRNA: 3'- agCGCCCaGau-UGCUUUUACcUGAGG- -5' |
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26256 | 5' | -49.1 | NC_005345.2 | + | 26236 | 0.69 | 0.854095 |
Target: 5'- -gGCGGcGUCU--CGGuucAGUGGAUUCCg -3' miRNA: 3'- agCGCC-CAGAuuGCUu--UUACCUGAGG- -5' |
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26256 | 5' | -49.1 | NC_005345.2 | + | 41107 | 0.7 | 0.824848 |
Target: 5'- aCGCGGGcCgcagcacgcccGCGguAcgGGGCUCCg -3' miRNA: 3'- aGCGCCCaGau---------UGCuuUuaCCUGAGG- -5' |
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26256 | 5' | -49.1 | NC_005345.2 | + | 11604 | 0.76 | 0.519291 |
Target: 5'- gCGUGGGgcggccgCUGGCGGGAGUGGGCg-- -3' miRNA: 3'- aGCGCCCa------GAUUGCUUUUACCUGagg -5' |
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26256 | 5' | -49.1 | NC_005345.2 | + | 47271 | 1.15 | 0.001902 |
Target: 5'- cUCGCGGGUCUAACGAAAAUGGACUCCa -3' miRNA: 3'- -AGCGCCCAGAUUGCUUUUACCUGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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