Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26257 | 3' | -53.4 | NC_005345.2 | + | 18406 | 0.66 | 0.809209 |
Target: 5'- aCCCucgccGGCcUCGCGCUCacggCCGUGAu -3' miRNA: 3'- -GGGcuuu-UCGaAGCGCGGGa---GGUACU- -5' |
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26257 | 3' | -53.4 | NC_005345.2 | + | 28086 | 0.66 | 0.789592 |
Target: 5'- aCCCGGAGGGU-----GCCgUCCGUGAg -3' miRNA: 3'- -GGGCUUUUCGaagcgCGGgAGGUACU- -5' |
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26257 | 3' | -53.4 | NC_005345.2 | + | 2913 | 0.66 | 0.789592 |
Target: 5'- aCCGGA----UUCGCGCCgCUCC-UGAc -3' miRNA: 3'- gGGCUUuucgAAGCGCGG-GAGGuACU- -5' |
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26257 | 3' | -53.4 | NC_005345.2 | + | 39015 | 0.66 | 0.789592 |
Target: 5'- cCCCGAAcugguGGCUgcuGCGCgUCUCCggGAa -3' miRNA: 3'- -GGGCUUu----UCGAag-CGCG-GGAGGuaCU- -5' |
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26257 | 3' | -53.4 | NC_005345.2 | + | 3275 | 0.66 | 0.789592 |
Target: 5'- cCCCGugcgcAGCUgCGUGCCgUCCAc-- -3' miRNA: 3'- -GGGCuuu--UCGAaGCGCGGgAGGUacu -5' |
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26257 | 3' | -53.4 | NC_005345.2 | + | 5241 | 0.66 | 0.779523 |
Target: 5'- gCCGAcgcGCUgCGCGCCg-CCGUGGc -3' miRNA: 3'- gGGCUuuuCGAaGCGCGGgaGGUACU- -5' |
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26257 | 3' | -53.4 | NC_005345.2 | + | 21006 | 0.66 | 0.779523 |
Target: 5'- uCCCGAGGGGCuUUCGCauaccCCCUCg---- -3' miRNA: 3'- -GGGCUUUUCG-AAGCGc----GGGAGguacu -5' |
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26257 | 3' | -53.4 | NC_005345.2 | + | 44655 | 0.66 | 0.769296 |
Target: 5'- uCCCGGucGGCUccucCGUGCCgUCCucgcUGAa -3' miRNA: 3'- -GGGCUuuUCGAa---GCGCGGgAGGu---ACU- -5' |
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26257 | 3' | -53.4 | NC_005345.2 | + | 2766 | 0.66 | 0.769296 |
Target: 5'- uUCGGAcggcguGGCUgcUCGCcgccGCCCUUCGUGAg -3' miRNA: 3'- gGGCUUu-----UCGA--AGCG----CGGGAGGUACU- -5' |
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26257 | 3' | -53.4 | NC_005345.2 | + | 47531 | 0.67 | 0.758922 |
Target: 5'- cCCCGGGAuGCUucccUCGUaGCCCUUCGa-- -3' miRNA: 3'- -GGGCUUUuCGA----AGCG-CGGGAGGUacu -5' |
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26257 | 3' | -53.4 | NC_005345.2 | + | 26359 | 0.67 | 0.748414 |
Target: 5'- cCCCGccGAGgaUCGCGCggaucCCUCCcgagGUGAc -3' miRNA: 3'- -GGGCuuUUCgaAGCGCG-----GGAGG----UACU- -5' |
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26257 | 3' | -53.4 | NC_005345.2 | + | 44879 | 0.67 | 0.745238 |
Target: 5'- gCCCGAGcuGCccgcaccgccggugUUCGCcggcaGCCCgagCCGUGAg -3' miRNA: 3'- -GGGCUUuuCG--------------AAGCG-----CGGGa--GGUACU- -5' |
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26257 | 3' | -53.4 | NC_005345.2 | + | 27967 | 0.67 | 0.727043 |
Target: 5'- aCCCGAAcGGCggUGCGCCgUCgAcGGg -3' miRNA: 3'- -GGGCUUuUCGaaGCGCGGgAGgUaCU- -5' |
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26257 | 3' | -53.4 | NC_005345.2 | + | 30135 | 0.68 | 0.672109 |
Target: 5'- gCCCG-AGGGCggCGCGCCaC-CCGUcGAg -3' miRNA: 3'- -GGGCuUUUCGaaGCGCGG-GaGGUA-CU- -5' |
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26257 | 3' | -53.4 | NC_005345.2 | + | 11053 | 0.68 | 0.649781 |
Target: 5'- aCCCGA--AGUUUCuGCGUgCgUCCAUGGc -3' miRNA: 3'- -GGGCUuuUCGAAG-CGCGgG-AGGUACU- -5' |
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26257 | 3' | -53.4 | NC_005345.2 | + | 46519 | 0.69 | 0.638586 |
Target: 5'- aCCCGGucAGUc-CGCGCCgCUCCAUc- -3' miRNA: 3'- -GGGCUuuUCGaaGCGCGG-GAGGUAcu -5' |
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26257 | 3' | -53.4 | NC_005345.2 | + | 28290 | 0.69 | 0.616188 |
Target: 5'- gUCGAcAAGC-UCGCGCCCgCCAaGGg -3' miRNA: 3'- gGGCUuUUCGaAGCGCGGGaGGUaCU- -5' |
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26257 | 3' | -53.4 | NC_005345.2 | + | 40830 | 0.7 | 0.582724 |
Target: 5'- gCCGGAAcGCUgcugcugCGCGCCg-CCAUGGc -3' miRNA: 3'- gGGCUUUuCGAa------GCGCGGgaGGUACU- -5' |
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26257 | 3' | -53.4 | NC_005345.2 | + | 15214 | 0.7 | 0.582724 |
Target: 5'- gUCGAAcgccuGCUUCGCGUCCUugaCCAUGc -3' miRNA: 3'- gGGCUUuu---CGAAGCGCGGGA---GGUACu -5' |
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26257 | 3' | -53.4 | NC_005345.2 | + | 29084 | 0.7 | 0.571642 |
Target: 5'- gCCCGggGugGGCUUCGagggCUCCGUGAa -3' miRNA: 3'- -GGGCuuU--UCGAAGCgcggGAGGUACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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