Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26259 | 5' | -51.4 | NC_005345.2 | + | 45492 | 0.66 | 0.844987 |
Target: 5'- gGCGAAGGUGGuGGGggCCGCGuccGCGa -3' miRNA: 3'- aCGCUUUCAUC-UUCuaGGCGUc--CGUa -5' |
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26259 | 5' | -51.4 | NC_005345.2 | + | 40251 | 0.66 | 0.826235 |
Target: 5'- gGCGAGcgGGgacGggGcgCCgGCGGGCAUg -3' miRNA: 3'- aCGCUU--UCau-CuuCuaGG-CGUCCGUA- -5' |
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26259 | 5' | -51.4 | NC_005345.2 | + | 24645 | 0.66 | 0.816511 |
Target: 5'- cGCGguGGUcGAccaGGAUcCCGCGGGCc- -3' miRNA: 3'- aCGCuuUCAuCU---UCUA-GGCGUCCGua -5' |
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26259 | 5' | -51.4 | NC_005345.2 | + | 16024 | 0.66 | 0.816511 |
Target: 5'- cGCGAGGGU--GAGAcCCGC-GGCGa -3' miRNA: 3'- aCGCUUUCAucUUCUaGGCGuCCGUa -5' |
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26259 | 5' | -51.4 | NC_005345.2 | + | 7730 | 0.68 | 0.754067 |
Target: 5'- -aCGA----AGGAGAUCCGCAGGUg- -3' miRNA: 3'- acGCUuucaUCUUCUAGGCGUCCGua -5' |
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26259 | 5' | -51.4 | NC_005345.2 | + | 31889 | 0.69 | 0.698098 |
Target: 5'- gUGCGGuGAGUucGggGAUCgGCGGGUg- -3' miRNA: 3'- -ACGCU-UUCAu-CuuCUAGgCGUCCGua -5' |
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26259 | 5' | -51.4 | NC_005345.2 | + | 423 | 0.69 | 0.698098 |
Target: 5'- aUGCGAGGGggcGGAcguaguacgggcGGGUCCGUGGcGCAUa -3' miRNA: 3'- -ACGCUUUCa--UCU------------UCUAGGCGUC-CGUA- -5' |
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26259 | 5' | -51.4 | NC_005345.2 | + | 6545 | 0.69 | 0.686636 |
Target: 5'- gGCGGGgcGGUGGggGAcguggcuaCGCGGGCGg -3' miRNA: 3'- aCGCUU--UCAUCuuCUag------GCGUCCGUa -5' |
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26259 | 5' | -51.4 | NC_005345.2 | + | 23463 | 0.69 | 0.686636 |
Target: 5'- gGCGAu-GUGGAuuGAgucgCCGCAGGCc- -3' miRNA: 3'- aCGCUuuCAUCUu-CUa---GGCGUCCGua -5' |
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26259 | 5' | -51.4 | NC_005345.2 | + | 26883 | 0.69 | 0.675116 |
Target: 5'- gUGCGGucgauGGGU-GAGGcgCUGCAGGCGg -3' miRNA: 3'- -ACGCU-----UUCAuCUUCuaGGCGUCCGUa -5' |
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26259 | 5' | -51.4 | NC_005345.2 | + | 34892 | 0.7 | 0.640334 |
Target: 5'- aGCGAcacGUuccGGggGAUCgUGCAGGCAg -3' miRNA: 3'- aCGCUuu-CA---UCuuCUAG-GCGUCCGUa -5' |
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26259 | 5' | -51.4 | NC_005345.2 | + | 19312 | 0.73 | 0.429309 |
Target: 5'- gUGCGAgggcuggaAAGUGGccGAUCUGCGGGCc- -3' miRNA: 3'- -ACGCU--------UUCAUCuuCUAGGCGUCCGua -5' |
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26259 | 5' | -51.4 | NC_005345.2 | + | 45692 | 0.74 | 0.380545 |
Target: 5'- cGCGAccGUGGAcAGGUCCcgGCGGGCGg -3' miRNA: 3'- aCGCUuuCAUCU-UCUAGG--CGUCCGUa -5' |
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26259 | 5' | -51.4 | NC_005345.2 | + | 34063 | 0.75 | 0.362103 |
Target: 5'- gGCGgcGGUAGGccuGAUCaCGCAGGCGc -3' miRNA: 3'- aCGCuuUCAUCUu--CUAG-GCGUCCGUa -5' |
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26259 | 5' | -51.4 | NC_005345.2 | + | 46836 | 1.07 | 0.002364 |
Target: 5'- uUGCGAAAGUAGAAGAUCCGCAGGCAUg -3' miRNA: 3'- -ACGCUUUCAUCUUCUAGGCGUCCGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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