Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26261 | 5' | -56.8 | NC_005345.2 | + | 19096 | 0.66 | 0.566814 |
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Target: 5'- cUCgCCgUCGGUGUCGUCgGCcCACg- -3' miRNA: 3'- cAGgGGgAGCUACAGCAG-CGaGUGau -5' |
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| 26261 | 5' | -56.8 | NC_005345.2 | + | 23193 | 0.66 | 0.555963 |
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Target: 5'- --aCCCgUCGAUGUCGUUGUgcCACa- -3' miRNA: 3'- cagGGGgAGCUACAGCAGCGa-GUGau -5' |
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| 26261 | 5' | -56.8 | NC_005345.2 | + | 10739 | 0.66 | 0.555963 |
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Target: 5'- -gCCgCgCUCGAUGUCGUCGCcgaCGCc- -3' miRNA: 3'- caGGgG-GAGCUACAGCAGCGa--GUGau -5' |
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| 26261 | 5' | -56.8 | NC_005345.2 | + | 16614 | 0.67 | 0.49239 |
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Target: 5'- -aCCCCCUCGgcGUCGgcaugcaCGCcgUCACUc -3' miRNA: 3'- caGGGGGAGCuaCAGCa------GCG--AGUGAu -5' |
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| 26261 | 5' | -56.8 | NC_005345.2 | + | 9389 | 0.69 | 0.404005 |
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Target: 5'- cUCCUCCUCGccGUCGUCGacggCGCg- -3' miRNA: 3'- cAGGGGGAGCuaCAGCAGCga--GUGau -5' |
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| 26261 | 5' | -56.8 | NC_005345.2 | + | 29203 | 0.7 | 0.35943 |
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Target: 5'- -gCCCCUUCGAgggcgcgcugcUGcUCGUCGuCUCGCUGc -3' miRNA: 3'- caGGGGGAGCU-----------AC-AGCAGC-GAGUGAU- -5' |
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| 26261 | 5' | -56.8 | NC_005345.2 | + | 3407 | 0.71 | 0.310637 |
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Target: 5'- aUCCCCCUCGAcgacgUGUCGgugUGgaCGCUGc -3' miRNA: 3'- cAGGGGGAGCU-----ACAGCa--GCgaGUGAU- -5' |
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| 26261 | 5' | -56.8 | NC_005345.2 | + | 16463 | 0.76 | 0.158444 |
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Target: 5'- uUCCCCCUCGGUGUCGacgccgggaucaucgUCcuGCUCGCg- -3' miRNA: 3'- cAGGGGGAGCUACAGC---------------AG--CGAGUGau -5' |
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| 26261 | 5' | -56.8 | NC_005345.2 | + | 44290 | 1.07 | 0.000839 |
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Target: 5'- aGUCCCCCUCGAUGUCGUCGCUCACUAg -3' miRNA: 3'- -CAGGGGGAGCUACAGCAGCGAGUGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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