miRNA: 3'- -CCaACUugGCCUa-------------GCUGACGCC-GGUG- -5'
miRNA: 3'- ccaaCUUGGC--CUAGC--UGACG------------CCGGUG- -5'
miRNA: 3'- -CCAaCU---UGGCC--UAGCUgaCGCCGGuG- -5'
miRNA: 3'- -CCAACU-UGGC-CUAGCu----GACGc-CGGUG- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26267 | 3' | -54.9 | NC_005345.2 | + | 19106 | 0.66 | 0.727944 |
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Target: 5'- uGGcUGAugUGGGcgcucaccgcauggUGGCUGCGGgCCGCa -3' miRNA: 3'- -CCaACUugGCCUa-------------GCUGACGCC-GGUG- -5' |
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| 26267 | 3' | -54.9 | NC_005345.2 | + | 4233 | 0.66 | 0.734322 |
|
Target: 5'- ---cGAGCCGauGGUCGccGCUGCggagcgggacgaGGCCACg -3' miRNA: 3'- ccaaCUUGGC--CUAGC--UGACG------------CCGGUG- -5' |
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| 26267 | 3' | -54.9 | NC_005345.2 | + | 7034 | 0.66 | 0.734322 |
|
Target: 5'- cGGUcGAccgGCCGGcgAUCGAg-GCGGCC-Cg -3' miRNA: 3'- -CCAaCU---UGGCC--UAGCUgaCGCCGGuG- -5' |
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| 26267 | 3' | -54.9 | NC_005345.2 | + | 44438 | 0.66 | 0.734322 |
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Target: 5'- cGGUUGGcACCGuGAUCGccgccCUGUucGCCGCa -3' miRNA: 3'- -CCAACU-UGGC-CUAGCu----GACGc-CGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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