miRNA: 3'- ccaaCUUGGcCUAGCUGACGCcGGUG- -5'
miRNA: 3'- -CCAa--CUugGCCUA-GCUgACGCCGGug -5'
miRNA: 3'- cCAACUUGgCCUagcUGACGCCGGUg -5'
miRNA: 3'- -CCa-ACUUGGCCUaGCUgaCGcCGGUG- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26267 | 3' | -54.9 | NC_005345.2 | + | 19412 | 0.7 | 0.474596 |
|
Target: 5'- ---cGAGCUcGGUCGGCUGCG-CCGCg -3' miRNA: 3'- ccaaCUUGGcCUAGCUGACGCcGGUG- -5' |
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| 26267 | 3' | -54.9 | NC_005345.2 | + | 31952 | 0.69 | 0.505602 |
|
Target: 5'- gGGUgcgGGugCGGGUgCGGgUGCGGCUg- -3' miRNA: 3'- -CCAa--CUugGCCUA-GCUgACGCCGGug -5' |
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| 26267 | 3' | -54.9 | NC_005345.2 | + | 28207 | 0.69 | 0.537445 |
|
Target: 5'- aGUUGGGCaGGGggaACUGCGGCCAa -3' miRNA: 3'- cCAACUUGgCCUagcUGACGCCGGUg -5' |
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| 26267 | 3' | -54.9 | NC_005345.2 | + | 10399 | 0.66 | 0.712929 |
|
Target: 5'- cGGcgUGAGCCGGG-CGAggGUcGCCACc -3' miRNA: 3'- -CCa-ACUUGGCCUaGCUgaCGcCGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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