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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26268 | 5' | -58.3 | NC_005345.2 | + | 40522 | 1.08 | 0.000455 |
|
Target: 5'- gAGCAGUCACGACUGCUCACCCCCGACc -3' miRNA: 3'- -UCGUCAGUGCUGACGAGUGGGGGCUG- -5' |
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| 26268 | 5' | -58.3 | NC_005345.2 | + | 40696 | 0.67 | 0.465924 |
|
Target: 5'- cGGgAGUCGCGACcgcgcccgUGCUCGUCgUCGACa -3' miRNA: 3'- -UCgUCAGUGCUG--------ACGAGUGGgGGCUG- -5' |
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| 26268 | 5' | -58.3 | NC_005345.2 | + | 41701 | 0.67 | 0.415698 |
|
Target: 5'- cGCGGUCACGAuccggucCUGCUCgacgucaACgCCCGuCg -3' miRNA: 3'- uCGUCAGUGCU-------GACGAG-------UGgGGGCuG- -5' |
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| 26268 | 5' | -58.3 | NC_005345.2 | + | 43406 | 0.67 | 0.456016 |
|
Target: 5'- cGCAGUCcuCGGCUGCg-GCCCggaacacggCCGGCc -3' miRNA: 3'- uCGUCAGu-GCUGACGagUGGG---------GGCUG- -5' |
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| 26268 | 5' | -58.3 | NC_005345.2 | + | 44095 | 0.71 | 0.244942 |
|
Target: 5'- cGCAG-CAC-ACcgGCUCGCCCUCGAUg -3' miRNA: 3'- uCGUCaGUGcUGa-CGAGUGGGGGCUG- -5' |
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| 26268 | 5' | -58.3 | NC_005345.2 | + | 45421 | 0.67 | 0.436549 |
|
Target: 5'- aGGCGG-CGCGggcgaacgaucaGCUGCaccUCGCCgCCGGCg -3' miRNA: 3'- -UCGUCaGUGC------------UGACG---AGUGGgGGCUG- -5' |
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| 26268 | 5' | -58.3 | NC_005345.2 | + | 45639 | 0.67 | 0.426996 |
|
Target: 5'- cGCAGuucgcUCAUGACgucCUCGCCUgCGACg -3' miRNA: 3'- uCGUC-----AGUGCUGac-GAGUGGGgGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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