Results 61 - 67 of 67 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26268 | 5' | -58.3 | NC_005345.2 | + | 5217 | 0.66 | 0.475942 |
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Target: 5'- gGGCAGcUCGCGGCcgGcCUgAUCgCCGACg -3' miRNA: 3'- -UCGUC-AGUGCUGa-C-GAgUGGgGGCUG- -5' |
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| 26268 | 5' | -58.3 | NC_005345.2 | + | 4950 | 0.69 | 0.33873 |
|
Target: 5'- uGGCGGUCACGgagacccacACgUGCUCGCaCUgCGACc -3' miRNA: 3'- -UCGUCAGUGC---------UG-ACGAGUG-GGgGCUG- -5' |
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| 26268 | 5' | -58.3 | NC_005345.2 | + | 4094 | 0.69 | 0.355283 |
|
Target: 5'- cGGUcgAG-CACGACggGCaCACCCCCGAg -3' miRNA: 3'- -UCG--UCaGUGCUGa-CGaGUGGGGGCUg -5' |
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| 26268 | 5' | -58.3 | NC_005345.2 | + | 3873 | 0.66 | 0.506612 |
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Target: 5'- cGGC-GUCccuGCG-CUGCUCuCCgCCGACg -3' miRNA: 3'- -UCGuCAG---UGCuGACGAGuGGgGGCUG- -5' |
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| 26268 | 5' | -58.3 | NC_005345.2 | + | 3537 | 0.74 | 0.155874 |
|
Target: 5'- cGGCGGUgACG-CUGUgacgaUCACCCUCGACc -3' miRNA: 3'- -UCGUCAgUGCuGACG-----AGUGGGGGCUG- -5' |
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| 26268 | 5' | -58.3 | NC_005345.2 | + | 2547 | 0.67 | 0.436549 |
|
Target: 5'- cGCGGUgGCGACgGCgagUGCCCggUCGACg -3' miRNA: 3'- uCGUCAgUGCUGaCGa--GUGGG--GGCUG- -5' |
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| 26268 | 5' | -58.3 | NC_005345.2 | + | 1149 | 0.72 | 0.209461 |
|
Target: 5'- uGGCGGUCGUGACUGCUCGgUCC-GGCu -3' miRNA: 3'- -UCGUCAGUGCUGACGAGUgGGGgCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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